Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity

被引：69

作者：

Vallee-Belisle, Alexis ^{[2
]}

Bonham, Andrew J. ^{[2
]}

Reich, Norbert O. ^{[2
,3
]}

Ricci, Francesco ^{[1
]}

Plaxco, Kevin W. ^{[2
,3
]}

机构：

[1] Univ Rome, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy

[2] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Interdept Program Biomol Sci & Engn, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 35期

关键词：

MOLECULAR BEACONS; PROTEINS; TARGETS; PROBES;

D O I：

10.1021/ja204775k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here "transcription factor beacons," binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-kappa B), and measure binding activity directly in crude nuclear extracts.

引用

页码：13836 / 13839

页数：4

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