H5N1 Whole-Virus Vaccine Induces Neutralizing Antibodies in Humans Which Are Protective in a Mouse Passive Transfer Model

被引：16

作者：

Howard, M. Keith ^{[1
]}

Sabarth, Nicolas ^{[1
]}

Savidis-Dacho, Helga ^{[1
]}

Portsmouth, Daniel ^{[1
]}

Kistner, Otfried ^{[1
]}

Kreil, Thomas R. ^{[1
]}

Ehrlich, Hartmut J. ^{[2
]}

Barrett, P. Noel ^{[1
]}

机构：

[1] Baxter BioSci, Vaccine Res & Dev, Orth, Austria

[2] Baxter BioSci, Global Res & Dev, Vienna, Austria

来源：

PLOS ONE | 2011年 / 6卷 / 08期

关键词：

PANDEMIC INFLUENZA VACCINE; CELL-CULTURE; MONOCLONAL-ANTIBODIES; THERAPEUTIC-EFFICACY; EGG-ADAPTATION; H1N1; VACCINE; WILD-TYPE; INFECTION; MICE; HEMAGGLUTININ;

D O I：

10.1371/journal.pone.0023791

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Vero cell culture-derived whole-virus H5N1 vaccines have been extensively tested in clinical trials and consistently demonstrated to be safe and immunogenic; however, clinical efficacy is difficult to evaluate in the absence of wide-spread human disease. A lethal mouse model has been utilized which allows investigation of the protective efficacy of active vaccination or passive transfer of vaccine induced sera following lethal H5N1 challenge. Methods: We used passive transfer of immune sera to investigate antibody-mediated protection elicited by a Vero cell-derived, non-adjuvanted inactivated whole-virus H5N1 vaccine. Mice were injected intravenously with H5N1 vaccine-induced rodent or human immune sera and subsequently challenged with a lethal dose of wild-type H5N1 virus. Results: Passive transfer of H5N1 vaccine-induced mouse, guinea pig and human immune sera provided dose-dependent protection of recipient mice against lethal challenge with wild-type H5N1 virus. Protective dose fifty values for serum H5N1 neutralizing antibody titers were calculated to be <= 1:11 for all immune sera, independently of source species. Conclusions: These data underpin the confidence that the Vero cell culture-derived, whole-virus H5N1 vaccine will be effective in a pandemic situation and support the use of neutralizing serum antibody titers as a correlate of protection for H5N1 vaccines.

引用

页数：7

共 49 条

[1] A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection [J].