From Csaba Horvath to Quality by Design: Visualizing Design Space in Selectivity Exploration of HPLC Separations

被引：19

作者：

Molnar, I. ^{[1
]}

Monks, K. E. ^{[1
]}

机构：

[1] Molnar Inst Appl Chromatog, D-10407 Berlin, Germany

来源：

CHROMATOGRAPHIA | 2011年 / 73卷

关键词：

Csaba Horvath; Solvophobic theory; Quality by Design; Design space; 3D computer modeling software; LIQUID-CHROMATOGRAPHY; RETENTION; ACIDS;

D O I：

10.1007/s10337-011-1968-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present paper starts by taking a look back at some of the pioneering work in high pressure liquid chromatography (HPLC) that went on in Csaba Horvath's laboratory in 1970s, through the eyes of I. Molnar. It then goes on to describe a very modern approach to HPLC method development within the Quality by Design framework: the multifactorial optimization of three critical HPLC method parameters, i.e. gradient time (t (G)), temperature (T), and ternary composition (B-1:B-2) based on 12 experiments. The effect of these experimental variables on critical resolution and selectivity was carried out in such a way as to systematically vary all three factors simultaneously.

引用

页码：S5 / S14

页数：10

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