Cotton leaf curl Multan virus βC1 Protein Induces Autophagy by Disrupting the Interaction of Autophagy-Related Protein 3 with Glyceraldehyde-3-Phosphate Dehydrogenases[OPEN]

被引：69

作者：

Ismayil, Asigul ^{[1
]}

Yang, Meng ^{[1
]}

Haxim, Yakupjan ^{[1
]}

Wang, Yunjing ^{[1
]}

Li, Jinlin ^{[1
]}

Han, Lu ^{[1
]}

Wang, Yan ^{[1
]}

Zheng, Xiyin ^{[1
]}

Wei, Xiang ^{[1
]}

Nagalakshmi, Ugrappa ^{[2
,3
]}

Hong, Yiguo ^{[4
]}

Hanley-Bowdoin, Linda ^{[5
]}

Liu, Yule ^{[1
]}

机构：

[1] Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Sch Life Sci, MOE Key Lab Bioinformat,Ctr Plant Biol, Beijing 100084, Peoples R China

[2] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA

[3] Univ Calif Davis, Coll Biol Sci, Genome Ctr, Davis, CA 95616 USA

[4] Hangzhou Normal Univ, Coll Life & Environm Sci, Res Ctr Plant RNA Signaling, Hangzhou 310036, Peoples R China

[5] North Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA

来源：

PLANT CELL | 2020年 / 32卷 / 04期

基金：

中国国家自然科学基金;

关键词：

CELL-DEATH; REGULATE AUTOPHAGY; IMMUNE-RESPONSE; DEGRADATION; COMPONENTS; BEGOMOVIRUS; MACHINERY; INFECTION; DISEASE; ATG6;

D O I：

10.1105/tpc.19.00759

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Viral beta C1 protein disrupts the ATG3-GAPC interaction and thereby induces autophagy. Autophagy plays an important role in plant-pathogen interactions. Several pathogens including viruses induce autophagy in plants, but the underpinning mechanism remains largely unclear. Furthermore, in virus-plant interactions, viral factor(s) that induce autophagy have yet to be identified. Here, we report that the beta C1 protein of Cotton leaf curl Multan betasatellite (CLCuMuB) interacts with cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC), a negative autophagic regulator, to induce autophagy in Nicotiana benthamiana. CLCuMuB beta C1 bound to GAPCs and disrupted the interaction between GAPCs and autophagy-related protein 3 (ATG3). A mutant beta C1 protein (beta C1(3A)) in which I45, Y48, and I53 were all substituted with Ala (A), had a dramatically reduced binding capacity with GAPCs, failed to disrupt the GAPCs-ATG3 interactions and failed to induce autophagy. Furthermore, mutant virus carrying beta C1(3A) showed increased symptoms and viral DNA accumulation associated with decreased autophagy in plants. These results suggest that CLCuMuB beta C1 activates autophagy by disrupting GAPCs-ATG3 interactions.

引用

页码：1124 / 1135

页数：12

共 47 条

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