Molecular Cloning and mRNA Expression of the Liver-Specific Cathepsin L1 Gene of the Olive Flounder, Paralichthys olivaceus

被引：6

作者：

Kim, Young-Ok ^{[1
]}

Park, Eun-Mi ^{[2
]}

Seo, Jung Soo ^{[3
]}

Nam, Bo-Hye ^{[1
]}

Kong, Hee Jeong ^{[1
]}

Kim, Woo-Jin ^{[1
]}

Kim, Bong-Seok ^{[1
]}

Kim, Kyung-Kil ^{[1
]}

Lee, Sang-Jun ^{[1
]}

机构：

[1] Natl Fisheries Res & Dev Inst, Biotechnol Res Div, Pusan 619705, South Korea

[2] Busan Reg Korea Food & Drug Adm, Imported Food Anal Div, Ctr Food & Drug Anal, Pusan 608829, South Korea

[3] Natl Fisheries Res & Dev Inst, Div Pathol, Pusan 619705, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2011年 / 75卷 / 06期

关键词：

Paralichthys olivaceus; cathepsin L; gene expression; RT-PCR; in situ hybridization; TROUT ONCORHYNCHUS-MYKISS; CARP CYPRINUS-CARPIO; CYSTEINE PROTEASES; PHYLOGENETIC-RELATIONSHIPS; WHITE MUSCLE; STARVATION; HEPATOPANCREAS; PURIFICATION; ENZYME; SALMON;

D O I：

10.1271/bbb.110220

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a homolog of cathepsin L from a cDNA library of the olive flounder liver. The flounder cathepsin L1 transcript consisted of 1,221 bp that encoded a polypeptide of 334 amino acids. The overall identity between flounder cathepsin L1 and other cathepsin Ls was 50-64%, and flounder cathepsin L1 contained the highly conserved ERFNIN-motif. A phylogenetic tree indicated that flounder cathepsin L1 is in the same monophyletic group as zebrafish cathepsin Lc. RT-PCR analysis revealed that cathepsin L1 transcripts were expressed only in the liver. They were detected from 28d post-hatching. Under starvation conditions, cathepsin L1 expression was decreased at 30d.

引用

页码：1214 / 1218

页数：5

共 26 条

[1]

AJ B, 2001, J BIOL CHEM, V382, P727

[2] Purification and characterization of cathepsin L from hepatopancreas of carp Cyprinus carpio [J].

Aranishi, F ;

Ogata, H ;

Hara, K ;

Osatomi, K ;

Ishihara, T .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1997, 118 (03) :531-537

[3] ALIGNMENT PHYLOGENY OF THE PAPAIN SUPERFAMILY OF CYSTEINE PROTEASES [J].

BERTI, PJ ;

STORER, AC .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 246 (02) :273-283

[4]

BOULAY CL, 1998, GENE, V218, P77

[5] Cysteine cathepsins: Cellular roadmap to different functions [J].

Brix, Klaudia ;

Dunkhorst, Anna ;

Mayer, Kristina ;

Jordans, Silvia .

BIOCHIMIE, 2008, 90 (02) :194-207

[6] Emerging roles for cysteine proteases in human biology [J].

Chapman, HA ;

Riese, RJ ;

Shi, GP .

ANNUAL REVIEW OF PHYSIOLOGY, 1997, 59 :63-88

[7] Molecular cloning and characterization of cathepsin L encoding genes from Fasciola gigantica [J].

Grams, R ;

Vichasri-Grams, S ;

Sobhon, P ;

Upatham, ES ;

Viyanant, V .

PARASITOLOGY INTERNATIONAL, 2001, 50 (02) :105-114

[8] Metabolic priorities during starvation:: enzyme sparing in liver and white muscle of Atlantic cod, Gadus morhua L. [J].

Guderley, H ;

Lapointe, D ;

Bédard, M ;

Dutil, JD .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2003, 135 (02) :347-356

[9] Cathepsin C from Schistosoma japonicum cDNA encoding the preproenzyme and its phylogenetic relationships [J].

Hola-Jamriska, L ;

Tort, JF ;

Dalton, JP ;

Day, SR ;

Fan, JJ ;

Aaskov, J ;

Brindley, PJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 255 (03) :527-534

[10] 2 DISTINCT GENE SUBFAMILIES WITHIN THE FAMILY OF CYSTEINE PROTEASE GENES [J].

KARRER, KM ;

PEIFFER, SL ;

DITOMAS, ME .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :3063-3067

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