Determination of Hormone Antagonists in Waste-Water Samples by Micellar Electrokinetic Chromatography

被引:3
作者
Rucins, Martins [1 ,2 ]
Baron, Daniel [1 ]
Plotniece, Aiva [2 ]
Petr, Jan [1 ]
机构
[1] Palacky Univ Olomouc, Reg Ctr Adv Technol & Mat, Dept Analyt Chem, 17 Listopadu 12, Olomouc 77146, Czech Republic
[2] Latvian Inst Organ Synth, Aizkraukles Str 21, LV-1006 Riga, Latvia
关键词
Endocrine disruptors; Micellar electrokinetic chromatography; Waste-water; ENDOCRINE-DISRUPTING COMPOUNDS; PERSONAL CARE PRODUCTS; CAPILLARY-ELECTROPHORESIS; PHARMACEUTICALS; ESTROGENS;
D O I
10.1007/s10337-018-3631-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The contamination of water represents a major problem in many parts of the world. Endocrine disrupting compounds belong to the water contaminants and affect, besides other things, hormonal equilibrium in the living organisms. Such compounds are of different chemical structures and there is a demand for their fast determination in water samples. In our work, we focused on the development of a simple and fast method for separation and determination of five model hormone antagonists, exemestane, toremifene, letrozole, anastrozole, and mifepristone, by micellar electrokinetic chromatography. Within the best conditions, 50mM sodium borate pH 9.5, 50mM SDS, 15% 1-propanol, 20kV, the separation of all the compounds lasted 8min. The method was successfully validated with LODs of 1.2-7.7 mu gmL(-1), LOQs of 4.0-25.6 mu gmL(-1) and intraday repeatability of peak areas of 0.8-1.1%. Moreover, it was applied to the analysis of spiked water samples from a local waste-water treatment plant using SPE as a pretreatment step.
引用
收藏
页码:1607 / 1612
页数:6
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