A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana

被引:5
作者
Kong, Mengmeng [1 ]
Wu, Yaozong [1 ]
Wang, Ziyuan [2 ]
Qu, Wantong [2 ]
Lan, Yixin [1 ]
Chen, Xin [1 ]
Liu, Yanyun [1 ]
Shahnaz, Perveen [1 ]
Yang, Zhongnan [2 ]
Yu, Qingbo [2 ]
Mi, Hualing [1 ]
机构
[1] Chinese Acad Sci, Natl Key Lab Plant Mol Genet, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai, Peoples R China
[2] Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai, Peoples R China
基金
中国国家自然科学基金;
关键词
Arabidopsis; chloroplast; plastid-encoded RNA polymerase; RpoA; RNA processing 8; PENTATRICOPEPTIDE REPEAT PROTEIN; ACTIVE CHROMOSOME COMPLEX; PLASTID SIGMA FACTORS; GENOME-WIDE ANALYSIS; POLYNUCLEOTIDE PHOSPHORYLASE; MESSENGER-RNA; REVEALS; POLYMERASES; INTERACTS; COMPONENT;
D O I
10.3389/fpls.2021.700975
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Chloroplast development involves the coordinated expression of both plastids- and nuclear-encoded genes in higher plants. However, the underlying mechanism still remains largely unknown. In this study, we isolated and characterized an Arabidopsis mutant with an albino lethality phenotype named RNA processing 8 (rp8). Genetic complementation analysis demonstrated that the gene AT4G37920 (RP8) was responsible for the mutated phenotype. The RP8 gene was strongly expressed in photosynthetic tissues at both transcription and translation protein levels. The RP8 protein is localized in the chloroplast and associated with the thylakoid. Disruption of the RP8 gene led to a defect in the accumulation of the rpoA mature transcript, which reduced the level of the RpoA protein, and affected the transcription of PEP-dependent genes. The abundance of the chloroplast rRNA, including 23S, 16S, 4.5S, and 5S rRNA, were reduced in the rp8 mutant, respectively, and the amounts of chloroplast ribosome proteins, such as, PRPS1(uS1c), PRPS5(uS5c), PRPL2 (uL2c), and PRPL4 (uL4c), were substantially decreased in the rp8 mutant, which indicated that knockout of RP8 seriously affected chloroplast translational machinery. Accordingly, the accumulation of photosynthetic proteins was seriously reduced. Taken together, these results indicate that the RP8 protein plays an important regulatory role in the rpoA transcript processing, which is required for the expression of chloroplast genes and chloroplast development in Arabidopsis.
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页数:13
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