Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel

被引:95
作者
Gaudet, Ian D. [1 ]
Shreiber, David I. [1 ]
机构
[1] Rutgers State Univ, Dept Biomed Engn, Piscataway, NJ 08854 USA
基金
美国国家科学基金会;
关键词
TISSUE ENGINEERING APPLICATIONS; CROSS-LINKING; MECHANICAL-PROPERTIES; STEM-CELLS; PEG HYDROGELS; GELS; BIOMATERIALS; GLUTARALDEHYDE; STABILIZATION; ENCAPSULATION;
D O I
10.1007/s13758-012-0025-y
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Type-I collagen is an attractive scaffold material for tissue engineering due to its ability to self-assemble into a fibrillar hydrogel, its innate support of tissue cells through bioactive adhesion sites, and its biodegradability. However, a lack of control of material properties has hampered its utility as a scaffold. We have modified collagen via the addition of methacrylate groups to create collagen methacrylamide (CMA) using a synthesis reaction that allows retention of fundamental characteristics of native collagen, including spontaneous fibrillar self-assembly and enzymatic biodegradability. This method allows for a rapid, five-fold increase in storage modulus upon irradiation with 365 nm light. Fibrillar diameter of CMA was not significantly different from native collagen. Collagenolytic degradability of uncrosslinked CMA was minimally reduced, while photocrosslinked CMA was significantly more resistant to degradation. Live/Dead staining demonstrated that a large majority (71%) of encapsulated mesenchymal stem cells remained viable 24 h after photocrosslinking, which further increased to 81% after 72 h. This material represents a novel platform for creating mechanically heterogeneous environments.
引用
收藏
页码:1 / 9
页数:9
相关论文
共 45 条
[1]   Stabilization of gelatin films by crosslinking with genipin [J].
Bigi, A ;
Cojazzi, G ;
Panzavolta, S ;
Roveri, N ;
Rubini, K .
BIOMATERIALS, 2002, 23 (24) :4827-4832
[2]   Photo-cross-linking of type I collagen gels in the presence of smooth muscle cells: Mechanical properties, cell viability, and function [J].
Brinkman, WT ;
Nagapudi, K ;
Thomas, BS ;
Chaikof, EL .
BIOMACROMOLECULES, 2003, 4 (04) :890-895
[3]   Encapsulating Chondrocytes in degrading PEG hydrogels with high modulus: Engineering gel structural changes to facilitate cartilaginous tissue production [J].
Bryant, SJ ;
Bender, RJ ;
Durand, KL ;
Anseth, KS .
BIOTECHNOLOGY AND BIOENGINEERING, 2004, 86 (07) :747-755
[4]   Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering [J].
Burdick, JA ;
Anseth, KS .
BIOMATERIALS, 2002, 23 (22) :4315-4323
[5]   Collagen tissue engineering: Development of novel biomaterials and applications [J].
Cen, Lian ;
Liu, Wei ;
Cui, Lei ;
Zhang, Wenjie ;
Cao, Yilin .
PEDIATRIC RESEARCH, 2008, 63 (05) :492-496
[6]   MECHANISM OF CROSSLINKING OF PROTEINS BY GLUTARALDEHYDE .3. REACTION WITH COLLAGEN IN TISSUES [J].
CHEUNG, DT ;
PERELMAN, N ;
KO, EC ;
NIMNI, ME .
CONNECTIVE TISSUE RESEARCH, 1985, 13 (02) :109-115
[7]   A critical review on polymer-based bio-engineered materials for scaffold development [J].
Cheung, Hoi-Yan ;
Lau, Kin-Tak ;
Lu, Tung-Po ;
Hui, David .
COMPOSITES PART B-ENGINEERING, 2007, 38 (03) :291-300
[8]   Cell-scaffold mechanical interplay within engineered tissue [J].
Dado, Dekel ;
Levenberg, Shulamit .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2009, 20 (06) :656-664
[9]   GLUTARALDEHYDE AS A CROSS-LINKING AGENT FOR COLLAGEN-BASED BIOMATERIALS [J].
DAMINK, LHHO ;
DIJKSTRA, PJ ;
VANLUYN, MJA ;
VANWACHEM, PB ;
NIEUWENHUIS, P ;
FEIJEN, J .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 1995, 6 (08) :460-472
[10]   In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide [J].
Damink, LHHO ;
Dijkstra, PJ ;
vanLuyn, MJA ;
vanWachem, PB ;
Nieuwenhuis, P ;
Feijen, J .
BIOMATERIALS, 1996, 17 (07) :679-684