Biochemical Analysis of the Role of Leucine-Rich Repeat Receptor-Like Kinases and the Carboxy-Terminus of Receptor Kinases in Regulating Kinase Activity in Arabidopsis thaliana and Brassica oleracea

被引:6
作者
Oh, Eun-Seok [1 ]
Lee, Yeon [1 ]
Chae, Won Byoung [2 ]
Rameneni, Jana Jeevan [3 ]
Park, Yong-Soon [1 ]
Lim, Yong Pyo [3 ]
Oh, Man-Ho [1 ]
机构
[1] Chungnam Natl Univ, Dept Biol Sci, Coll Biol Sci & Biotechnol, Daejeon 34134, South Korea
[2] RDA, Natl Inst Hort & Herbal Sci, Vegetable Res Div, Wonju 55365, South Korea
[3] Chungnam Natl Univ, Dept Hort, Coll Agr & Life Sci, Daejeon 34134, South Korea
来源
MOLECULES | 2018年 / 23卷 / 01期
基金
新加坡国家研究基金会;
关键词
Arabidopsis thaliana; brassinosteroid; Brassica oleracea; leucine-rich repeat receptor-like kinases; phosphorylation; PHOSPHORYLATION SITES; RESIDUES; COMPLEX;
D O I
10.3390/molecules23010236
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein post-translational modification by phosphorylation is essential for the activity and stability of proteins in higher plants and underlies their responses to diverse stimuli. There are more than 300 leucine-rich repeat receptor-like kinases (LRR-RLKs), a major group of receptor-like kinases (RLKs) that plays an important role in growth, development, and biotic stress responses in higher plants. To analyze auto- and transphosphorylation patterns and kinase activities in vitro, 43 full-length complementary DNA (cDNA) sequences were cloned from genes encoding LRR-RLKs. Autophosphorylation activity was found in the cytoplasmic domains (CDs) of 18 LRR-RLKs; 13 of these LRR-RLKs with autophosphorylation activity showed transphosphorylation in Escherichia coli. BRI1-Associated Receptor Kinase (BAK1), which is critically involved in the brassinosteroid and plant innate immunity signal transduction pathways, showed strong auto- and transphosphorylation with multi-specific kinase activity within 2 h of induction of Brassica oleraceae BAK1-CD (BoBAK1-CD) in E. coli; moreover, the carboxy-terminus of LRR-RLKs regulated phosphorylation and kinase activity in Arabidopsis thaliana and vegetative crops.
引用
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页数:10
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