Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins

被引：43

作者：

Li, Jie ^{[1
,2
]}

Chen, Zhenbin ^{[1
,2
,3
]}

Di, Duolong ^{[3
]}

机构：

[1] Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Gansu, Peoples R China

[2] Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou 730050, Gansu, Peoples R China

[3] Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China

来源：

FOOD CHEMISTRY | 2012年 / 132卷 / 01期

关键词：

Separation; Rebaudioside A; Stevia Rebaudiana Bertoni; Steviosides; Macroporous adsorption resin mixed bed; CARBON; ACID; STEVIOSIDE; GLYCOSIDE; KINETICS;

D O I：

10.1016/j.foodchem.2011.10.077

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The separation and purification of Rebaudioside A (RA) from Stevia Rebaudiana Bertoni crude extracts (Steviosides) by macroporous adsorption resin (MAR) mixed bed were systematically investigated. MAR mixed bed of HPD750-LSA40-LSA30-DS401 was selected due to its better separation degree. Based on the kinetics/thermodynamics experiment of the mixed bed, it was found that the experimental data fitted better to the pseudo-second-order model, and intra-particle diffusion was rate-limiting step. The adsorption isotherm was consistent with IV equilibrium adsorption isotherm classified by Brunauer. Furthermore, the influencing factors for the separation of RA based on HPLC were also investigated. Under the optimal conditions, the separation degree for RA (D-AS) increased from 0.771 to 1.54. Moreover, the experimental results showed that the purity of the obtained product increased from 60% to 97%. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：268 / 276

页数：9

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