Cellular biosensors for drug discovery

被引：32

作者：

Durick, K ^{[1
]}

Negulescu, P ^{[1
]}

机构：

[1] Aurora Biosci Corp, San Diego, CA 92121 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 7-8期

关键词：

cell biology; fluorescent probe chemistry; miniaturization; automation;

D O I：

10.1016/S0956-5663(01)00173-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Recent advances in cell biology, fluorescent probe chemistry, miniaturization and automation have allowed the use of mammalian cells in a variety of medical and industrial applications. Here we describe the generation of cell-based biosensors, engineered to optically report specific biological activity. Cellular biosensors are comprised of living cells and can be used in various applications, including screening chemical libraries for drug discovery and environmental sensing. Panels of biosensors may also be useful for elucidating the function of novel genes. Here we describe two examples of the construction and use of engineered cell lines as biosensors for drug discovery. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：587 / 592

页数：6

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