Printed Carbon Microelectrodes for Electrochemical Detection of Single Vesicle Release from PC12 Cells

被引：24

作者：

Yakushenko, Alexey

Schnitker, Jan

Wolfrum, Bernhard ^{[1
]}

机构：

[1] Forschungszentrum Julich, Peter Grunberg Inst PGI ICS 8 8, D-52425 Julich, Germany

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 10期

关键词：

EXOCYTOTIC EVENTS; FUSION PORE; AMPEROMETRY; ELECTRODES; ARRAYS; CATECHOLAMINES; SECRETION; CHEMISTRY; SYSTEMS; PASTE;

D O I：

10.1021/ac300460s

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a disposable system for recording neurotransmitter release from individual cells in vitro. A simple yet reliable microelectrode fabrication process is introduced using screen-printed carbon paste. It allows rapid fabrication of devices at low costs without standard clean-room technology. We demonstrate functionality of the system by real-time observation of vesicle release from single PC12 (rat pheochromocytoma) cells. The cells are cultured directly on the chip and can be used for immediate or long-term in vitro experiments. Thus, our approach may serve as a platform for pharmacological cell culture studies.

引用

收藏

页码：4613 / 4617

页数：5

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