Transgressive segregation reveals mechanisms of Arabidopsis immunity to Brassica-infecting races of white rust (Albugo candida)

被引：39

作者：

Cevik, Volkan ^{[1
,2
]}

Boutrot, Freddy ^{[1
]}

Apel, Wiebke ^{[1
,3
]}

Robert-Seilaniantz, Alexandre ^{[1
,4
]}

Furzer, Oliver J. ^{[1
,5
]}

Redkar, Amey ^{[1
,6
]}

Castel, Baptiste ^{[1
]}

Kover, Paula X. ^{[2
]}

Prince, David C. ^{[1
,7
]}

Holub, Eric B. ^{[8
]}

Jones, Jonathan D. G. ^{[1
]}

机构：

[1] Univ East Anglia, Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

[2] Univ Bath, Dept Biol & Biochem, Milner Ctr Evolut, Bath BA2 7AY, Avon, England

[3] Humboldt Univ, Inst Biol, Expt Biophys, D-10115 Berlin, Germany

[4] Univ Rennes, INRA, Agrocampus Ouest, Inst Genet Environm & Plant Protect, F-35650 Le Rheu, France

[5] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA

[6] Univ Cordoba, Dept Genet, E-14071 Cordoba, Spain

[7] Univ East Anglia, Sch Biol Sci, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England

[8] Univ Warwick, Sch Life Sci, Warwick Crop Ctr, Wellesbourne CV35 9EF, Warwick, England

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2019年 / 116卷 / 07期

基金：

欧洲研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

Arabidopsis thaliana; oomycete; Albugo candida; nonhost resistance; Brassicaceae; NONHOST RESISTANCE; TRIGGERED IMMUNITY; PATHOGEN EFFECTOR; PLANT IMMUNITY; CELL-DEATH; FLAX RUST; GENE; PROTEIN; RECOGNITION; THALIANA;

D O I：

10.1073/pnas.1812911116

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Arabidopsis thaliana accessions are universally resistant at the adult leaf stage to white rust (Albugo candida) races that infect the crop species Brassica juncea and Brassica oleracea. We used transgressive segregation in recombinant inbred lines to test if this apparent species-wide (nonhost) resistance in A. thaliana is due to natural pyramiding of multiple Resistance (R) genes. We screened 593 inbred lines from an Arabidopsis multiparent advanced generation intercross (MAGIC) mapping population, derived from 19 resistant parental accessions, and identified two transgressive segregants that are susceptible to the pathogen. These were crossed to each MAGIC parent, and analysis of resulting F2 progeny followed by positional cloning showed that resistance to an isolate of A. candida race 2 (Ac2V) can be explained in each accession by at least one of four genes encoding nucleotide-binding, leucine-rich repeat (NLR) immune receptors. An additional gene was identified that confers resistance to an isolate of A. candida race 9 (AcBoT) that infects B. oleracea. Thus, effector-triggered immunity conferred by distinct NLR-encoding genes in multiple A. thaliana accessions provides species-wide resistance to these crop pathogens.

引用

页码：2767 / 2773

页数：7

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