High performance liquid chromatography reverse phase method for determination of Nitazomanide from Pharmaceutical Formulations (Oral Suspension and Tablets)

被引：0

作者：

Narayan, Harlikar Jayvant ^{[1
]}

Mahendra, Amlani Arun ^{[1
]}

机构：

[1] Wilson Coll, Dept Chem, Bombay 400007, Maharashtra, India

来源：

RESEARCH JOURNAL OF CHEMISTRY AND ENVIRONMENT | 2007年 / 11卷 / 03期

关键词：

high performance liquid chromatography; reverse phase; Nitazoxanide;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The aim of the study is to develop a high performance liquid chromatography (HPLC) assay method for rapid and accurate determination of Nitazoxanide from Pharmaceutical Formulations (Oral Suspension and Tablets). The experimental procedure involved Diode Array defector (at 240nm), mobile phase (0.005M tetra-butyl ammonium hydrogen sulphate and acetonitrile in the volume ratio of 35:65 with the flow rate of 1.0 mL per minute) and an Inertsil ODS 3V C-18 (250 mm x 4.6 mm, 5 mu size) column. A calibration curve of mean peak area vs. concentration showed good linearity within the LOQ (0.2%) and 180% of the test range. The recoveries ranged from 99% to 102%. The % RSD for Intra day and Inter day was = 2.0 for both oral suspension as well as tablets. Excipients present in the dosage forms did not show any interference with the Nitazoxanide.

引用

页码：42 / 46

页数：5