Supplementation of chilling storage medium with glutathione protects rooster sperm quality

被引：21
作者：
Masoudi, R. [1 ]
Sharafi, M. [2 ]
Pourazadi, L. [2 ]
Davachi, N. Dadashpour [3 ]
Asadzadeh, N. [1 ]
Esmaeilkhanian, S. [1 ]
Dirandeh, E. [4 ]
机构：
[1] Agr Res Educ & Extens Org AREEO, Anim Sci Res Inst Iran ASRI, Karaj, Iran
[2] Tarbiat Modares Univ, Dept Poultry Sci, Fac Agr, Tehran, Iran
[3] Agr Res Educ & Extens Org AREEO, Dept Res Breeding & Prod Lab Anim, Razi Vaccine & Serum Res Inst, Karaj, Iran
[4] Sari Agr Sci & Nat Resources Univ, Dept Anim Sci, Sari, Iran
来源：
CRYOBIOLOGY | 2020年 / 92卷
关键词：
Chilling; Fertility; Fresh semen; GSH; Rooster; REDUCED GLUTATHIONE; CRYOPRESERVATION; SPERMATOZOA; MOTILITY;
D O I：
10.1016/j.cryobiol.2019.10.005
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
This study was aimed to evaluate the effect of addition of reduced glutathione (GSH) to the extender on the rooster's semen quality parameters and fertility potential. Semen samples were diluted with Lake extender contained 0, 0.5, 1, 2, 4 and 8 mM GSH. Then, were chilled to 5 degrees C and stored for a period of 48 h. Sperm motion characteristics, viability, membrane integrity, lipid peroxidation, mitochondrial activity and fertility potential were evaluated. At the initiation of the experiment (0 h), GSH did not affect sperm parameters, while 2-4 mM GSH improved (P <= 0.05) quality indicators during storage periods. Moreover, the samples treated with 2-4 mM GSH have had a lower lipid peroxidation compared to other groups (P <= 0.05). Artificial insemination using the semen samples, which had been stored in groups treated with 2-4 mM GSH for a period of 24 h, led to greater (P <= 0.05) fertilizing potential compared to the control group.
引用
收藏
页码：260 / 262
页数：3
相关论文
共 19 条
[1]   Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism [J].
Armstrong, JS ;
Rajasekaran, M ;
Chamulitrat, W ;
Gatti, P ;
Hellstrom, WJ ;
Sikka, SC .
FREE RADICAL BIOLOGY AND MEDICINE, 1999, 26 (7-8) :869-880
[2]   Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen [J].
Aurich, JE ;
Schonherr, U ;
Hoppe, H ;
Aurich, C .
THERIOGENOLOGY, 1997, 48 (02) :185-192
[3]   Thiols prevent H2O2-mediated loss of sperm motility in cryopreserved bull semen [J].
Bilodeau, JF ;
Blanchette, S ;
Gagnon, C ;
Sirard, MA .
THERIOGENOLOGY, 2001, 56 (02) :275-286
[4]   Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen [J].
Bucak, Mustafa N. ;
Tekin, Necmettin .
SMALL RUMINANT RESEARCH, 2007, 73 (1-3) :103-108
[5]   L-Carnitine in rooster semen cryopreservation: Flow cytometric, biochemical and motion findings for frozen-thawed sperm [J].
Fattah, A. ;
Sharafi, M. ;
Masoudi, R. ;
Shahverdi, A. ;
Esmaeili, V. ;
Najafi, A. .
CRYOBIOLOGY, 2017, 74 :148-153
[6]   Decrease in glutathione content in boar sperm after cryopreservation -: Effect of the addition of reduced glutathione to the freezing and thawing extenders [J].
Gadea, J ;
Sellés, E ;
Marco, MA ;
Coy, P ;
Matás, C ;
Romar, R ;
Ruiz, S .
THERIOGENOLOGY, 2004, 62 (3-4) :690-701
[7]   Reduced glutathione content in human sperm is decreased after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders [J].
Gadea, J. ;
Molla, M. ;
Selles, E. ;
Marco, M. A. ;
Garcia-Vazquez, F. A. ;
Gardon, J. C. .
CRYOBIOLOGY, 2011, 62 (01) :40-46
[8]   An effective method for improving the fertility of glycerol-exposed poultry semen [J].
Long, JA ;
Kulkarni, G .
POULTRY SCIENCE, 2004, 83 (09) :1594-1601
[9]   Improvement of rooster semen quality using coenzyme Q10 during cooling storage in the Lake extender [J].
Masoudi, R. ;
Sharafi, M. ;
Pourazadi, L. .
CRYOBIOLOGY, 2019, 88 :87-91
[10]   Effects of reduced glutathione on the quality of rooster sperm during cryopreservation [J].
Masoudi, R. ;
Sharafi, M. ;
Shahneh, A. Zare ;
Khodaei-Motlagh, M. .
THERIOGENOLOGY, 2019, 128 :149-155
12