Long-term stability of cancer biomarkers in human serum: biomarkers of oxidative stress and redox status, homocysteine, CRP and the enzymes ALT and GGT

被引：34

作者：

Jansen, Eugene ^{[1
]}

Beekhof, Piet ^{[1
]}

Viezeliene, Dale ^{[2
]}

Muzakova, Vladimira ^{[3
]}

Skalicky, Jiri ^{[4
]}

机构：

[1] Natl Inst Publ Hlth & Environm, Ctr Hlth Protect, NL-3720 BA Bilthoven, Netherlands

[2] Lithuanian Univ Hlth Sci, Med Acad, Dept Biochem, LT-44307 Kaunas, Lithuania

[3] Univ Pardubice, Fac Chem Technol, Dept Biol & Biochem Sci, Pardubice, Czech Republic

[4] Reg Hosp Pardubice, Dept Clin Biochem & Diagnost, Pardubice, Czech Republic

来源：

BIOMARKERS IN MEDICINE | 2015年 / 9卷 / 05期

关键词：

gamma-glutamyltransferase; alanine aminotransferase; cancer biomarkers; C-reactive protein; homocysteine; hydroperoxides; stability; storage; total thiols; C-REACTIVE PROTEIN; GAMMA-GLUTAMYL-TRANSFERASE; CORONARY-ARTERY-DISEASE; COLORECTAL-CANCER; ALANINE AMINOTRANSFERASE; CARDIOVASCULAR-DISEASE; ANTIOXIDANT ACTIVITY; PLASMA HOMOCYSTEINE; BLOOD HOMOCYSTEINE; BREAST-CANCER;

D O I：

10.2217/bmm.15.14

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aim: Five frequently used biomarkers in cancer research and epidemiological studies were tested for their assay stability upon storage of serum for 12 months at -20 and -70/-80 degrees C. Materials & methods: The biomarker assays include reactive oxygen metabolites (ROM), the total thiol levels (TTL), homocysteine (HCy), C-reactive protein (HS-CRP) and two liver enzymes, alanine aminotransferase (ALT) and -glutamyltransferase (GGT). Results: The assays for ROM, HCy, HS-CRP and GGT were stable in human serum samples at the two temperatures tested. The two other assays TTL and ALT, however, showed statistically significant differences in their stability between -20 and -80 degrees C. Conclusion: Therefore, storage at -80 degrees C is advised to maintain a reliable assay outcome when serum samples have to be stored for longer periods.

引用

页码：425 / 432

页数：8

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