Optimization of leucrose production by dextransucrase from Streptococcus mutans and its application as an adipogenesis regulator

被引：10

作者：

Lee, Daeyeon ^{[1
,2
]}

Lee, Jihye ^{[3
]}

Hong, Moon-Gi ^{[4
]}

Lee, Byung-Hoo ^{[4
]}

Kim, Young-Min ^{[5
]}

Chang, Pahn-Shick ^{[6
,7
]}

Kim, Yuri ^{[3
]}

Yoo, Sang-Ho ^{[1
,2
]}

机构：

[1] Sejong Univ, Dept Food Sci & Biotechnol, 209 Neungdong Ro, Seoul 05006, South Korea

[2] Sejong Univ, Carbohydrate Bioprod Res Ctr, 209 Neungdong Ro, Seoul 05006, South Korea

[3] Ewha Womans Univ, Dept Nutr Sci & Food Management, 52 Ewhayeodae Gil, Seoul 03760, South Korea

[4] Gachon Univ, Dept Food Sci & Biotechnol, Seongnam 13120, South Korea

[5] Chonnam Natl Univ, Dept Biotechnol, Gwangju 61186, South Korea

[6] Seoul Natl Univ, Dept Agr Biotechnol, Ctr Food & Bioconvergence, Seoul 08826, South Korea

[7] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea

来源：

JOURNAL OF FUNCTIONAL FOODS | 2017年 / 39卷

基金：

新加坡国家研究基金会;

关键词：

Leucrose; Sucrose; Dextransucrase; Streptococcus mutans; Adipogenesis; ADIPOCYTE DIFFERENTIATION; TURANOSE PRODUCTION; HIGH GLUCOSE; OBESITY; RECEPTOR; CELLS; POLYPHENOLS; SUPPRESSES; EXPRESSION; ALPHA;

D O I：

10.1016/j.jff.2017.10.019

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Leucrose is a sucrose isomer which has an alpha-1,5-linkage, and slowly hydrolyzed to glucose and fructose by small intestinal alpha-glucosidases. Leucrose can be produced by an isomerization reaction of dextransucrase on a sucrose substrate. In this study, the recombinant dextransucrase from Streptococcus mutans (SmDS) was applied to optimize the reaction conditions for leucrose production. With a substrate mixture of 0.5 M sucrose + 1.0 M fructose, the greatest yield (ca. 24.5%) of leucrose was obtained by SIDS treatment at 30 degrees C for 120 h. When preadipocyte 3T3-L1 cells were treated with leucrose, this disaccharide inhibited intracellular lipid accumulation in a dose-dependent manner and significantly suppressed mRNA levels of major adipogenic genes, including CCAT/enhancer-binding protein alpha (C/EBP alpha), peroxisome proliferator-activated receptor-gamma (PPAR gamma), fatty acid synthase (FAS), and sterol regulatory element-binding protein-1C (SREBP-1C). Phosphorylation of P13 kinase/Akt/mTOR was also reduced with leucrose treatment. These results suggest that leucrose has a potential in regulating adipogenesis.

引用

页码：238 / 244

页数：7

共 36 条

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