Direct analysis of pharmaceutical compounds in human plasma with chromatographic resolution using an alkyl-bonded silica rod column

被引：65

作者：

Plumb, R ^{[1
]}

Dear, G ^{[1
]}

Mallet, D ^{[1
]}

Ayrton, J ^{[1
]}

机构：

[1] GlaxoSmithKline, DMPK Div, Ware SG12 0DP, Herts, England

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2001年 / 15卷 / 12期

关键词：

D O I：

10.1002/rcm.329

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monolithic columns have been successfully used with steep gradient and high flow rates for the direct analysis of a candidate pharmaceutical compound in human plasma. The monolithic columns showed excellent robustness with nearly 300 20-muL injections of plasma (diluted 1:1 with water) being made onto one column without significant deterioration in performance. The system gave excellent sensitivity with a limit of quantification of 5 ng/mL being achieved. Unlike previous methods of direct analysis the monolithic columns showed excellent resolution even after nearly 300 plasma injections. The column performance was measured before and after the analysis of the plasma samples. Copyright (C) 2001 John Wiley & Sons, Ltd.

引用

页码：986 / 993

页数：8