CpG methylation in cell-free Epstein-Barr virus DNA in patients with EBV-Hodgkin lymphoma

被引：11

作者：

Shamay, Meir ^{[1
,2
]}

Kanakry, Jennifer A. ^{[1
,3
]}

Low, John S. W. ^{[4
]}

Horowitz, Netanel A. ^{[5
]}

Journo, Guy ^{[2
]}

Ahuja, Anuj ^{[2
]}

Eran, Yonatan ^{[2
]}

Barzilai, Elinor ^{[5
]}

Dann, Eldad J. ^{[5
,6
]}

Stone, Jennifer ^{[1
]}

Woo, Wan Lu ^{[4
]}

Hsieh, Wen-Son ^{[4
]}

Xian, Rena R. ^{[1
,7
]}

Ambinder, Richard F. ^{[1
]}

机构：

[1] Johns Hopkins Sch Med, Dept Oncol, 389 CRB1,1650 Orleans St, Baltimore, MD 21287 USA

[2] Bar Ilan Univ, Azrieli Fac Med, Daniella Lee Casper Lab Viral Oncol, Safed, Israel

[3] NCI, Expt Transplantat & Immunol Branch, NIH, Bethesda, MD 20892 USA

[4] Natl Univ Singapore, Canc Sci Inst Singapore, Singapore, Singapore

[5] Rambam Hlth Care Campus, Dept Hematol & Bone Marrow Transplantat, Haifa, Israel

[6] Technion, Bruce Rappaport Fac Med, Haifa, Israel

[7] Johns Hopkins Sch Med, Dept Pathol, Baltimore, MD 21287 USA

来源：

BLOOD ADVANCES | 2020年 / 4卷 / 08期

基金：

美国国家卫生研究院; 新加坡国家研究基金会;

关键词：

LYMPHOPROLIFERATIVE DISEASE; PLASMA;

D O I：

10.1182/bloodadvances.2020001511

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Epstein-Barr virus (EBV) is associated with a variety of tumors and nonmalignant conditions. Latent EBV genomes in cells, including tumor cells, are often CpG methylated, whereas virion DNA is not CpG methylated. We demonstrate that methyl CpG binding magnetic beads can be used to fractionate among sources of EBV DNA (DNA extracted from laboratory-purified virions vs DNA extracted from latently infected cell lines). We then applied the technique to plasma specimens and showed that this technique can distinguish EBV DNA from patients with EBV-associated tumors (nasopharyngeal carcinoma, Hodgkin lymphoma) and viral DNA from patients without EBV-associated tumors, including immunocompromised patients and patients with EBV(-) Hodgkin lymphoma.

引用

页码：1624 / 1627

页数：4

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