BMI1 enables interspecies chimerism with human pluripotent stem cells

被引：40

作者：

Huang, Ke ^{[1
,2
,3
,4
]}

Zhu, Yanling ^{[1
,2
,3
,4
,5
]}

Ma, Yanlin ^{[6
]}

Zhao, Bentian ^{[1
,2
]}

Fan, Nana ^{[1
,2
]}

Li, Yuhang ^{[1
,2
,5
]}

Song, Hong ^{[1
,2
]}

Chu, Shilong ^{[1
,2
]}

Ouyang, Zhen ^{[1
,2
]}

Zhang, Quanjun ^{[1
,2
]}

Xing, Qi ^{[1
,2
]}

Lai, Chengdan ^{[1
,2
]}

Li, Nan ^{[1
,2
]}

Zhang, Tian ^{[1
,2
,5
]}

Gu, Jiaming ^{[1
,2
,5
]}

Kang, Baoqiang ^{[1
,2
]}

Shan, Yongli ^{[1
,2
]}

Lai, Keyu ^{[1
,2
]}

Huang, Wenhao ^{[1
,2
]}

Mai, Yuchan ^{[1
,2
]}

Wang, Qing ^{[1
,2
]}

Li, Jinbing ^{[1
,2
]}

Lin, Aiping ^{[1
,2
]}

Zhang, Yanqi ^{[1
,2
]}

Zhong, Xiaofen ^{[1
,2
]}

Liao, Baojian ^{[1
,2
,4
]}

Lai, Liangxue ^{[1
,2
]}

Chen, Jiekai ^{[1
,2
]}

Pei, Duanqing ^{[1
,2
]}

Pan, Guangjin ^{[1
,2
,3
,4
]}

机构：

[1] Guangzhou Med Univ, Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Key Lab Regenerat Biol,Joint Sch Life Sci, Guangzhou 510530, Guangdong, Peoples R China

[2] Chinese Acad Sci, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou 510530, Guangdong, Peoples R China

[3] Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing 100101, Peoples R China

[4] Chinese Acad Sci, Hefei Inst Stem Cell & Regenerat Med, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China

[5] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[6] Hainan Med Univ, Affiliated Hosp 1, Hainan Prov Key Lab Human Reprod Med & Genet Res, Haikou 570102, Hainan, Peoples R China

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

中国国家自然科学基金;

关键词：

DERIVATION; INDUCTION; STATE; DIFFERENTIATION; ESTABLISHMENT; INHIBITION; APOPTOSIS; CIRCUITRY; CULTURE; LINES;

D O I：

10.1038/s41467-018-07098-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human pluripotent stem cells (hPSCs) exhibit very limited contribution to interspecies chimeras. One explanation is that the conventional hPSCs are in a primed state and so unable to form chimeras in pre-implantation embryos. Here, we show that the conventional hPSCs undergo rapid apoptosis when injected into mouse pre-implantation embryos. While, forced-expression of BMI1, a polycomb factor in hPSCs overcomes the apoptosis and enables hPSCs to integrate into mouse pre-implantation embryos and subsequently contribute to chimeras with both embryonic and extra-embryonic tissues. In addition, BMI1 also enables hPSCs to integrate into pre-implantation embryos of other species, such as rabbit and pig. Notably, BMI1 high expression and anti-apoptosis are also indicators for naive hPSCs to form chimera in mouse embryos. Together, our findings reveal that the apoptosis is an initial barrier in interspecies chimerism using hPSCs and provide a rational to improve it.

引用

页数：11

共 37 条

[1] Derivation of pluripotent epiblast stem cells from mammalian embryos [J].

Brons, I. Gabrielle M. ;

Smithers, Lucy E. ;

Trotter, Matthew W. B. ;

Rugg-Gunn, Peter ;

Sun, Bowen ;

de Sousa Lopes, Susana M. Chuva ;

Howlett, Sarah K. ;

Clarkson, Amanda ;

Ahrlund-Richter, Lars ;

Pedersen, Roger A. ;

Vallier, Ludovic .

NATURE, 2007, 448 (7150) :191-U7

[2] A Murine ESC-like State Facilitates Transgenesis and Homologous Recombination in Human Pluripotent Stem Cells [J].

Buecker, Christa ;

Chen, Hsu-Hsin ;

Polo, Jose Maria ;

Daheron, Laurence ;

Bu, Lei ;

Barakat, Tahsin Stefan ;

Okwieka, Patricia ;

Porter, Andrew ;

Gribnau, Joost ;

Hochedlinger, Konrad ;

Geijsen, Niels .

CELL STEM CELL, 2010, 6 (06) :535-546

[3] Induction of a Human Pluripotent State with Distinct Regulatory Circuitry that Resembles Preimplantation Epiblast [J].

Chan, Yun-Shen ;

Goeke, Jonathan ;

Ng, Jia-Hui ;

Lu, Xinyi ;

Gonzales, Kevin Andrew Uy ;

Tan, Cheng-Peow ;

Tng, Wei-Quan ;

Hong, Zhong-Zhi ;

Lim, Yee-Siang ;

Ng, Huck-Hui .

CELL STEM CELL, 2013, 13 (06) :663-675

[4] Hallmarks of pluripotency [J].

De Los Angeles, Alejandro ;

Ferrari, Francesco ;

Xi, Ruibin ;

Fujiwara, Yuko ;

Benvenisty, Nissim ;

Deng, Hongkui ;

Hochedlinger, Konrad ;

Jaenisch, Rudolf ;

Lee, Soohyun ;

Leitch, Harry G. ;

Lensch, M. William ;

Lujan, Ernesto ;

Pei, Duanqing ;

Rossant, Janet ;

Wernig, Marius ;

Park, Peter J. ;

Daley, George Q. .

NATURE, 2015, 525 (7570) :469-478

[5] ESTABLISHMENT IN CULTURE OF PLURIPOTENTIAL CELLS FROM MOUSE EMBRYOS [J].

EVANS, MJ ;

KAUFMAN, MH .

NATURE, 1981, 292 (5819) :154-156

[6] Derivation of novel human ground state naive pluripotent stem cells [J].

Gafni, Ohad ;

Weinberger, Leehee ;

Mansour, Abed AlFatah ;

Manor, Yair S. ;

Chomsky, Elad ;

Ben-Yosef, Dalit ;

Kalma, Yael ;

Viukov, Sergey ;

Maza, Itay ;

Zviran, Asaf ;

Rais, Yoach ;

Shipony, Zohar ;

Mukamel, Zohar ;

Krupalnik, Vladislav ;

Zerbib, Mirie ;

Geula, Shay ;

Caspi, Inbal ;

Schneir, Dan ;

Shwartz, Tamar ;

Gilad, Shlomit ;

Amann-Zalcenstein, Daniela ;

Benjamin, Sima ;

Amit, Ido ;

Tanay, Amos ;

Massarwa, Rada ;

Novershtern, Noa ;

Hanna, Jacob H. .

NATURE, 2013, 504 (7479) :282-+

[7] Naive Pluripotent Stem Cells Derived Directly from Isolated Cells of the Human Inner Cell Mass [J].

Guo, Ge ;

von Meyenn, Ferdinand ;

Santos, Fatima ;

Chen, Yaoyao ;

Reik, Wolf ;

Bertone, Paul ;

Smith, Austin ;

Nichols, Jennifer .

STEM CELL REPORTS, 2016, 6 (04) :437-446

[8] Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs [J].

Hanna, Jacob ;

Cheng, Albert W. ;

Saha, Krishanu ;

Kim, Jongpil ;

Lengner, Christopher J. ;

Soldner, Frank ;

Cassady, John P. ;

Muffat, Julien ;

Carey, Bryce W. ;

Jaenisch, Rudolf .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (20) :9222-9227

[9] Human pluripotent stem cells as a model of trophoblast differentiation in both normal development and disease [J].

Horii, Mariko ;

Li, Yingchun ;

Wakeland, Anna K. ;

Pizzo, Donald P. ;

Nelson, Katharine K. ;

Sabatini, Karen ;

Laurent, Louise Chang ;

Liu, Ying ;

Parast, Mana M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (27) :E3882-E3891

[10] In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation [J].

Huang, Yali ;

Osorno, Rodrigo ;

Tsakiridis, Anestis ;

Wilson, Valerie .

CELL REPORTS, 2012, 2 (06) :1571-1578

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