Cannabinoid CB1 receptor expression and affinity in the rat hippocampus following bilateral vestibular deafferentation

被引：3

作者：

Baek, Jean Ha ^{[1
]}

Zheng, Yiwen ^{[1
]}

Darlington, Cynthia L. ^{[1
]}

Smith, Paul F. ^{[1
]}

机构：

[1] Univ Otago, Sch Med, Sch Med Sci, Dept Pharmacol & Toxicol, Dunedin, New Zealand

来源：

NEUROSCIENCE LETTERS | 2011年 / 487卷 / 03期

关键词：

Cannabinoid receptors; Hippocampus; Vestibular lesions; Rat brain; MEDIAL TEMPORAL-LOBE; LONG-TERM CHANGES; SYSTEM; CHANNELS; REQUIRE; BINDING; LESIONS; DAMAGE;

D O I：

10.1016/j.neulet.2010.10.050

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Numerous studies have shown that bilateral vestibular deafferentation (BVD) results in spatial memory deficits and hippocampal dysfunction in rats and humans. Since cannabinoid CB1 receptors are well known to regulate synaptic plasticity in the hippocampus, we investigated whether BVD resulted in changes in CB1 receptor expression and affinity in the rat hippocampus at 1,3 and 7 days post-surgery, using a combination of Western blotting and radioligand binding. Using Western blotting, we found that CB1 receptor expression was significantly lower in BVD animals compared to sham controls only in the CA3 area across the 3 time points (P = 0.03). CB1 receptor expression decreased significantly over time for both the BVD and sham animals (P = 0.000). The radioligand binding assays showed no significant change in the IC50 of the CB1 receptor for the cannabinoid CB1/CB2 receptor agonist, WIN55,212-2. These results suggest that the CB1 receptor down-regulates in the CA3 region of the hippocampus following BVD, but with no changes in the affinity of the CBI receptor for WIN55,212-2. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：330 / 334

页数：5

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