Reverse-phase protein microarrays for tissue-based analysis

被引:0
作者
Speer, R
Wulfkuhle, JD
Liotta, LA
Petricoin, EF [1 ]
机构
[1] NCI, Ctr Canc Res, FDA NCI Clin Proteom Program, Lab Pathol,NIH, Bethesda, MD 20892 USA
[2] George Mason Univ, Ctr Appl Proteom & Mol Med, Dept Mol & Microbiol, Manassas, VA 20110 USA
关键词
microarrays; protein proteomics; reverse phase; tailored therapy;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The deciphering of the human genome has elucidated our biological structural design and has generated insights into disease development and pathogenesis. At the same time, knowledge of genetic changes during disease processes has demonstrated the need to move beyond genomics towards proteomics and a systems biology approach to science. Analyzing the proteome comprises more than just a numeration of proteins. In fact, it characterizes proteins within cells in the context of their functional status and interactions in their physiological micro- and macro-environments. As dysregulated signaling often underpins most human diseases, an overarching goal of proteomics is to profile the working state of signaling pathways, to develop 'circuit maps' of normal and diseased protein networks and identify hyperactive, defective or inoperable transduction pathways. Reverse-phase protein microarrays represent a new technology that can generate a multiplex readout of dozens of phosphorylated events simultaneously to profile the state of a signaling pathway target even after the cell is lyzed and the contents denatured.
引用
收藏
页码:240 / 245
页数:6
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