Thermoresponsive poly[tri(ethylene glycol) monoethyl ether methacrylate]-peptide surfaces obtained by radiation grafting-synthesis and characterisation

被引：15

作者：

Adamus, A. ^{[1
]}

Komasa, J. ^{[1
]}

Kadlubowski, S. ^{[1
]}

Ulanski, P. ^{[1
]}

Rosiak, J. M. ^{[1
]}

Kawecki, M. ^{[2
]}

Klama-Baryla, A. ^{[2
]}

Dworak, A. ^{[3
]}

Trzebicka, B. ^{[3
]}

Szweda, R. ^{[3
]}

机构：

[1] Lodz Univ Technol, Inst Appl Radiat Chem, Wroblewskiego 15, PL-93590 Lodz, Poland

[2] Ctr Burn Treatment, Jana Pawla 2, PL-41100 Siemianowice Slaskie, Poland

[3] Polish Acad Sci, Ctr Polymer & Carbon Mat, M Curie Sklodowskiej 34, PL-41819 Zabrze, Poland

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2016年 / 145卷

关键词：

Thermoresponsive surfaces; Radiation grafting; Poly[tri(ethylene glycol)monoethyl ether methacrylate; Surface modification; Peptides promoting cells' adhesion; IKVAV; POLYMER BRUSHES; MEDIATED POLYMERIZATION; ADHESION PEPTIDES; ETHYL ETHER; POLYPROPYLENE; TEMPERATURE; METHACRYLATE; EXPOSURE; FILMS;

D O I：

10.1016/j.colsurfb.2016.04.050

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This report demonstrates the feasibility of radiation grafting for the preparation of polymer layers functionalised with short peptide ligands which promote cell adhesion. Thermoresponsive poly [tri(ethylene glycol) monoethyl ether methacrylate] (PTEGMA) layers were synthesised on a polypropylene substrate by post-irradiation grafting. A cell adhesion moiety, the CF-IKVAVK peptide modified with a methacrylamide function and a fluorescent label were introduced to the surface during the polymerisation process. The amount of CF-IKVAVK was easily controlled by changing its concentration in the reaction mixture. The changes in the surface composition, morphology, philicity and thickness at each step of the polypropylene functionalisation confirmed that the surface modification procedures were successful. The increase in environmental temperature above the cloud point temperature of PTEGMA caused a decrease in surface philicity. The obtained PTEGMA and PTEGMA-peptide surfaces above Tcp were tested as scaffolds for fibroblast sheet culture and temperature induced detachment. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：185 / 193

页数：9

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