Validation of the Microreader 28A ID System: A 6-dye multiplex amplification assay for forensic application

被引：3

作者：

Yin, Lu ^{[1
]}

Zhu, Jing ^{[2
]}

Qu, Shengqiu ^{[1
]}

Li, Yifan ^{[3
]}

Liu, Yuqing ^{[4
]}

Yu, Zailiang ^{[5
]}

Yang, Fan ^{[5
]}

Zhang, Ranran ^{[1
]}

Xue, Jiaming ^{[1
]}

Huang, Sicheng ^{[6
]}

Liang, Weibo ^{[1
]}

机构：

[1] Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 608 Forens Bldg 16,South Renming Rd,Sect 3, CN-610041 Chengdu, Sichuan, Peoples R China

[2] Sichuan Police Coll, Dept Forens Med, Luzhou, Sichuan, Peoples R China

[3] Beijing Microread Genet Co Ltd, Beijing, Peoples R China

[4] Sichuan Univ, Lab Metabon & Gynecol Dis Res, West China Second Univ Hosp, Chengdu, Sichuan, Peoples R China

[5] Suzhou Microread Genet, Suzhou, Peoples R China

[6] Chengdu Publ Secur Bur, Dept Forens Genet, Inst Forens Sci, Chengdu, Sichuan, Peoples R China

来源：

ELECTROPHORESIS | 2021年 / 42卷 / 19期

关键词：

6-Dye multiplex PCR; CE; Forensic science; Microreader 28A ID System; Validation; CODIS CORE LOCI; SHADOW BANDS; DNA; IDENTIFICATION; PCR;

D O I：

10.1002/elps.202100110

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Microreader 28A ID System is a new 28-plex genotyping system with 6-dye multiplex amplification, which allows the simultaneous amplification of all 20 Combined DNA Index System (CODIS) core loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, vWA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, D22S1045), plus five extended STRs loci (D6S1043, Penta D, Penta E, DYS391, SE33), 2 Y-Indels (Rs2032678, Rs771783753), and the amelogenin loci. This system can be used for forensic analyses, such as personal identification, kinship testing, scientific research, database applications, and other aspects of human genetic identification. The validation of the Microreader 28A ID System followed the "Validation Guidelines for DNA Analysis Methods (2016)" described by the Scientific Working Group on DNA Analysis Methods and the regulations published by the China Ministry of Public Security. Our tests included PCR-based studies, sensitivity study, precision and accuracy evaluation, stutter percentage and heterozygous peak height ratio, inhibitor tests, species specificity, and population studies. The validation results suggest that the Microreader 28A ID system is a robust and reliable amplification kit for personal identification, kinship testing, and forensic database applications.

引用

页码：1928 / 1935

页数：8

共 19 条

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