Proteome analysis of human pancreatic ductal adenocarcinoma tissue using two-dimensional gel electrophoresis and tandem mass spectrometry for identification of disease-related proteins

被引：21

作者：

Tian, Rui ^{[1
]}

Wei, Li-Ming ^{[2
]}

Qin, Ren-Yi ^{[1
]}

Li, Yan ^{[3
]}

Du, Zhi-Yong ^{[1
]}

Xia, Wei ^{[1
]}

Shi, Cheng-Jian ^{[1
]}

Jin, Hong ^{[4
]}

机构：

[1] Huazhong Univ Sci & Technol, Affiliated Tongji Hosp, Tongji Med Coll, Dept Biliary Pancreat Surg, Wuhan 430030, Peoples R China

[2] Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China

[3] Huazhong Univ Sci & Technol, Affiliated Union Hosp, Tongji Med Coll, Dept Dermatol, Wuhan 430030, Peoples R China

[4] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

来源：

DIGESTIVE DISEASES AND SCIENCES | 2008年 / 53卷 / 01期

基金：

中国国家自然科学基金;

关键词：

pancreatic cancer; proteome; two-dimensional gel electrophoresis; mass spectrometry;

D O I：

10.1007/s10620-007-9823-3

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

A comparative proteomic approach has been used to identify and analyze proteins related to pancreatic cancer. Proteomes of eight pairs of clinical pancreatic ductal adenocarcinoma (PDAC) tissue samples and samples of normal adjacent tissue were obtained by two-dimensional gel electrophoresis (2DE). Comprehensive analysis of proteins was focused on total protein spots for which there were statistical differences between the two groups. Proteins were identified by peptide mass fingerprinting with tandem mass spectrometry (MS-MS). Western blotting and immunohistochemistry (IHC) were also performed to verify the expression of some candidate proteins. Thirty protein spots were identified, including proteases, antioxidant proteins, signal-transduction proteins, calcium-binding proteins, structural proteins, chaperones, and others. Western blotting and IHC confirmed up-regulated expression of two candidate proteins, nucleotide diphosphatase kinase (NDPK) and annexin II, in tumorous tissues. These results suggest that combination of 2DE with MS is an effective strategy for discovery of differently expressed proteins in PDAC which may be molecular markers for diagnosis or therapeutic targets.

引用

页码：65 / 72

页数：8

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