Electrogenerated chemiluminescence aptamer-based biosensor for the determination of cocaine

被引:132
作者
Li, Yan [1 ]
Qi, Honglan [1 ]
Peng, Yage [1 ]
Yang, Jia [1 ]
Zhang, Chengxiao [1 ]
机构
[1] Shaanxi Normal Univ, Sch Chem & Mat Sci, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China
基金
中国国家自然科学基金;
关键词
biosensor; aptamer; electrogenerated chemiluminescence; cocaine; tris (2,2'-bipyridyl) ruthenium derivatives;
D O I
10.1016/j.elecom.2007.07.038
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
A novel electrogenerated chemiluminescence aptamer-based (ECL-AB) biosensor for the determination of a small molecule drug is designed employing cocaine-binding aptamer as molecular recognition element for cocaine as a model analyte and ruthenium complex served as an ECL label. A 5'-terminal cocaine-binding aptamer with the ECL label at 3'-terminal of the aptamer was utilized as an ECL probe. The ECL-AB biosensors were fabricated by immobilizing the ECL probe onto a gold electrode surface via thiol-Au interactions. An enhanced ECL signal is generated upon recognition of the target cocaine, attributed to a change in the conformation of the ECL probe from random coil-like configuration on the probe-modified film to three-way junction structure, in close proximity to the sensor interface. The integrated ECL intensity versus the concentration of cocaine was linear in the range from 5.0 x 10(-9) to 3.0 x 10(-7) M. The detection limit was 1.0 x 10(-9) M. This work demonstrates that the combination of a highly binding aptamer to analyte with a highly sensitive ECL technique to design ECL-AB biosensor is a great promising approach for the determination of small molecule drugs. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:2571 / 2575
页数:5
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