Influence of substrate stiffness on cell-substrate interfacial adhesion and spreading: A mechano-chemical coupling model

被引:29
作者
Huang, Jianyong
Peng, Xiaoling
Xiong, Chunyang [1 ]
Fang, Jing
机构
[1] Peking Univ, Dept Biomed Engn, Beijing 100871, Peoples R China
关键词
Cell-substrate interaction; Substrate stiffness; Adhesion strength; Spreading area; Mechano-chemical coupling model; TRACTION FORCES; MECHANICS; DYNAMICS; RIGIDITY; CHEMISTRY; SURFACES; KINETICS; CONTACT; SOFT;
D O I
10.1016/j.jcis.2010.12.055
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Cell interactions with extracellular matrix, such as cell adhesion and spreading, are crucial for many biological functions and processes. Recent experimental progresses have demonstrated that substrate rigidities exert a remarkable influence on cell-substrate interfacial adhesion and spreading behaviors. The underlying biophysical mechanism, however, remains elusive. Based on the classical Bell-Dembo's theory, this paper develops a mechano-chemical coupling model to physically describe cell adhesion and spreading mediated by substrate stiffness. By investigating the competitive nature between cell-substrate specific attraction and non-specific repulsion, the kinetic relation of receptor-ligand interplay is established, in which the influences of receptor-ligand separation, substrate elasticity and non-specific repulsion on cell adhesions are especially addressed. According to mechanical equilibrium conditions between cell membranes and underlying elastic substrates, an analytical expression is then deduced to relate the cell-substrate interfacial adhesion strength to the substrate rigidity. Moreover, by means of the conventional wetting theory, the dependence of steady-state cell spreading on substrate stiffness is also quantitatively studied. Comparisons with the existing experimental data show that the proposed model can be used to explore cell-substrate interactions regulated by substrate rigidities. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:503 / 508
页数:6
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