Effect of an avidin-biotin binding system on chondrocyte adhesion, growth and gene expression

被引：34

作者：

Tsai, WB ^{[1
]}

Wang, MC ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Chem Engn, Taipei 106, Taiwan

来源：

BIOMATERIALS | 2005年 / 26卷 / 16期

关键词：

biotin; avidin; chondrocytes; cell adhesion; cell proliferation; gene expression;

D O I：

10.1016/j.biomaterials.2004.08.014

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Cell adhesion to synthetic biomaterials is a prerequisite for anchorage cell culture and tissue engineering. The current study investigated utilization of an avidin-biotin binding system in enhancing chondrocyte adhesion to tissue culture polystyrene (TCPS). Biotinylated chondrocytes adhered to avidin-coated TCPS more quickly than untreated chondrocytes to bare TCPS. Also the avidin-biotin binding system enhanced cell initial spreading. However, the effects were only transient. The growth of biotinylated chondrocytes was first decreased during the first 3 days but increased afterwards. The progeny of biotinylated chondrocytes still maintained the ability in expressing cartilage extracellular matrix proteins such as type II collagen, type IX collagen and aggrecan. These results show potential for the application of the avidin-biotin binding system to cell culture and tissue engineering. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：3141 / 3151

页数：11

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