Esculetin induces apoptosis and inhibits adipogenesis in 3T3-L1 cells

被引:72
|
作者
Yang, Jeong-Yeh
Della-Fera, Mary Anne
Hartzell, Diane L.
Nelson-Dooley, Cass
Hausman, Dorothy B.
Baile, Clifton A.
机构
[1] Univ Georgia, Dept Anim & Dairy Sci, Athens, GA 30602 USA
[2] Univ Georgia, Dept Foods & Nutr, Athens, GA 30602 USA
关键词
esculletin; adipogenesis; apoptosis; 3T3-L1; adipocytes; polyphenol;
D O I
10.1038/oby.2006.194
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: To determine the effects of esculetin, a plant V phenolic compound with apoptotic activity in cancer cells, on 3T3-L1 adipocyte apoptosis and adipogenesis. Research Methods and Procedures: 3T3-L1 pre-confluent preadipocytes and lipid-filled adipocytes were incubated with esculetin (0 to 800 mu M) for up to 48 hours. Viability was determined using the Cell Titer 96 Aqueous One Solution cell proliferation assay; apoptosis was quantified by measurement of single-stranded DNA. Post-confluent preadipocytes were incubated with esculetin for up to 6 days during maturation. Adipogenesis was quantified by measuring lipid content using Nile Red dye; cells were also stained with Oil Red 0 for visual confirmation of effects on lipid accumulation. Results: In mature adipocytes, esculetin caused a time- and dose-related increase in adipocyte apoptosis and a decrease in viability. Apoptosis was increased after only 6 hours by 400 and 800 mu M esculetin (p < 0.05), and after 48 hours, as little as 50 mu M esculetin increased apoptosis (p < 0.05). In preadipocytes, apoptosis was detectable only after 48 hours (p < 0.05) with 200 mu M esculetin and higher concentrations. However, results of the cell viability assay indicated a reduction in preadipocyte number in a time- and dose-related manner, beginning as early as 6 hours with 400 and 800 mu M esculetin (p < 0.05). Esculetin also inhibited adipogenesis of 3T3-L1 preadipocytes. Esculetin-mediated inhibition of adipocyte differentiation occurred during the early, intermediate, and late stages of the differentiation process. In addition, esculetin induced apoptosis during the late stage of differentiation. Discussion: These findings suggest that esculetin can alter fat cell number by direct effects on cell viability, adipogenesis, and apoptosis in 3T3-L1 cells.
引用
收藏
页码:1691 / 1699
页数:9
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