ESBL/pAmpC-Producing Escherichia coli Causing Urinary Tract Infections in Non-Related Companion Animals and Humans

被引：13
作者：
Belas, Adriana [1 ,2 ,3 ]
Marques, Catia [1 ,2 ,3 ]
Menezes, Juliana [1 ,2 ]
da Gama, Luis Telo [1 ,2 ]
Cavaco-Silva, Patricia [4 ,5 ]
Pomba, Constanca [1 ,2 ]
机构：
[1] Univ Lisbon, Fac Vet Med, Ctr Interdisciplinary Res Anim Hlth CIISA, P-1300477 Lisbon, Portugal
[2] Associate Lab Anim & Vet Sci AL4Anim, P-1300477 Lisbon, Portugal
[3] Lusofona Univ, Fac Vet Med, P-1749024 Lisbon, Portugal
[4] Inst Univ Egas Moniz, Ctr Invest Interdisciplinar Egas Moniz, P-2829511 Caparica, Portugal
[5] Technophage, P-1649028 Lisbon, Portugal
来源：
ANTIBIOTICS-BASEL | 2022年 / 11卷 / 05期
关键词：
Escherichia coli; ESBL; AmpC; pathogenicity; companion animals; humans; urinary tract infection; SEQUENCE TYPE 131; BETA-LACTAMASE GENES; EXTENDED-SPECTRUM; ANTIMICROBIAL RESISTANCE; MOLECULAR EPIDEMIOLOGY; MULTIPLEX PCR; KLEBSIELLA-PNEUMONIAE; VIRULENCE GENES; PUBLIC-HEALTH; CLONAL GROUPS;
D O I：
10.3390/antibiotics11050559
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Urinary tract infections (UTI) caused by Escherichia coli are frequently diagnosed in humans and companion animals. Extended-spectrum beta-lactamase (ESBL)- and cephalosporinase (pAmpC)-producing Escherichia coli are worldwide-disseminated and frequently multidrug-resistant, hence leading to treatment failure and public health concerns. This study aimed to characterize and compare ESBL/pAmpC-producing E. coli strains causing community-acquired UTI in companion animals and non-related humans. Third-generation cephalosporin (3GC)-resistant E. coli (companion animals n = 35; humans n = 85) isolated from patients with UTI were tested against 14 antimicrobials following CLSI guidelines. PCR-based assays were used to detect the major E. coli phylogenetic groups, pathogenicity associated-islands (PAIs), virulence genes, and ESBLs/pAmpC resistance genes. ESBL/pAmpC-producing E. coli isolates were typed by multi-locus sequence typing (MLST) and PCR. E. coli strains from companion animals and humans shared two MDR high-risk clonal lineages: ST131 and ST648. To the best of our knowledge, this study reports the first description of E. coli ST131 clade C1-M27 and the clonal lineage ST131 clade A in humans with community-acquired UTI in Portugal. Considering that companion animals with UTI are generally treated at home by the owners, measures should be implemented to avoid the spread of multidrug-resistant high-risk clones to humans and their household environment.
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页数：15
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