Inhibitors of phosphatidylinositol 3-kinase and mTOR but not Akt enhance replication of bovine ephemeral fever virus

被引：8

作者：

Ji, Wen T. ^{[2
]}

Wang, Ying C. ^{[3
]}

Lin, Feng L. ^{[2
,4
]}

Liao, Ming H. ^{[2
]}

Shih, Wen L. ^{[1
,3
]}

Liu, Hung J. ^{[1
,2
]}

机构：

[1] Natl Chung Hsing Univ, Inst Mol Biol, Taichung 402, Taiwan

[2] Natl Pingtung Univ Sci & Technol, Dept Vet Med, Pingtung 912, Taiwan

[3] Natl Pingtung Univ Sci & Technol, Grad Inst Biotechnol, Pingtung 912, Taiwan

[4] Tajen Univ Sci & Technol, Dept Pharm, Pingtung 912, Taiwan

来源：

VETERINARY JOURNAL | 2011年 / 187卷 / 01期

关键词：

Bovine ephemeral fever virus; Phosphatidylinositol; 3-kinase; Akt; mTOR; Inhibitors; Viral replication; PHOSPHOINOSITIDE; 3-KINASE; ACTIVATION; TRANSCRIPTION; TRANSLATION; WORTMANNIN; MECHANISM; RAPAMYCIN; SURVIVAL; PATHWAY;

D O I：

10.1016/j.tvjl.2009.11.003

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Non-segmented, negative-sense RNA viruses (NNSVs) depend on Akt (protein kinase B) for efficient replication. Infection with bovine ephemeral fever virus (BEFV) increases Akt phosphorylation. This study examined the effect of inhibition of phosphatidylinositol 3-kinase (PI3K)-Akt signalling on BEFV replication, since PI3K is the major upstream regulator of Akt. Treatment of BEFV-infected cells with two specific PI3K inhibitors (wortmannin and LY294002) enhanced replication of BEFV when compared to the effects of Akt inhibitors III and IV. BEFV antagonised the effects of PI3K inhibitors on Akt dephosphorylation. Inhibition of mTOR by rapamycin also enhanced replication of BEFV. The results provide evidences that inhibition of PI3K and mTOR has positive effects on replication of BEFV. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：119 / 123

页数：5

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