Five challenges to bringing single-molecule force spectroscopy into living cells

被引：128

作者：

Dufrene, Yves F. ^{[1
]}

Evans, Evan ^{[2
]}

Engel, Andreas ^{[3
,4
]}

Helenius, Jonne ^{[5
]}

Gaub, Hermann E. ^{[6
]}

Mueller, Daniel J. ^{[5
]}

机构：

[1] Catholic Univ Louvain, Inst Condensed Matter & Nanosci, B-1348 Louvain, Belgium

[2] Boston Univ, Boston, MA 02215 USA

[3] Case Western Reserve Univ, Dept Pharmacol, Cleveland, OH 44106 USA

[4] Univ Basel, Biozentrum, Basel, Switzerland

[5] Tech Hsch Zurich, Dept Biosyst Sci & Engn, Basel, Switzerland

[6] Univ Munich, Munich, Germany

来源：

NATURE METHODS | 2011年 / 8卷 / 02期

关键词：

TOOLBOX;

D O I：

10.1038/nmeth0211-123

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In recent years, single-molecule force spectroscopy techniques have been used to study how inter- and intramolecular interactions control the assembly and functional state of biomolecular machinery in vitro. Here we discuss the problems and challenges that need to be addressed to bring these technologies into living cells and to learn how cellular machinery is controlled in vivo. © 2011 Nature America, Inc. All rights reserved.

引用

页码：123 / 127

页数：5

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