West Nile Virus and Tick-Borne Encephalitis Virus Are Endemic in Equids in Eastern Austria

被引：14

作者：

de Heus, Phebe ^{[1
]}

Kolodziejek, Jolanta ^{[2
]}

Hubalek, Zdenek ^{[3
]}

Dimmel, Katharina ^{[2
]}

Racher, Victoria ^{[4
]}

Nowotny, Norbert ^{[2
,5
]}

Cavalleri, Jessika-M, V ^{[1
]}

机构：

[1] Univ Vet Med Vienna, Clin Unit Equine Internal Med, Vet Pl 1, A-1210 Vienna, Austria

[2] Univ Vet Med Vienna, Inst Virol, Viral Zoonoses Emerging & Vector Borne Infect Grp, Vet Pl 1, A-1210 Vienna, Austria

[3] Czech Acad Sci, Inst Vertebrate Biol, Kvetna 8, Brno 60365, Czech Republic

[4] Univ Salzburg, Dept Math, Hellbrunner Str 34, A-5020 Salzburg, Austria

[5] Mohammed Bin Rashid Univ Med & Hlth Sci, Coll Med, Dept Basic Med Sci, Bldg 14,POB 505055, Dubai, U Arab Emirates

来源：

VIRUSES-BASEL | 2021年 / 13卷 / 09期

关键词：

flavivirus; West Nile virus; tick-borne encephalitis virus; Usutu virus; seroprevalence; horses; epidemiology; Austria; USUTU-VIRUS; LINEAGE; 2; SEROLOGICAL EVIDENCE; HORSES; INFECTION; FLAVIVIRUS; EUROPE; SEROPREVALENCE; SEROSURVEY; EMERGENCE;

D O I：

10.3390/v13091873

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The emergence of West Nile virus (WNV) and Usutu virus (USUV) in addition to the autochthonous tick-borne encephalitis virus (TBEV) in Europe causes rising concern for public and animal health. The first equine case of West Nile neuroinvasive disease in Austria was diagnosed in 2016. As a consequence, a cross-sectional seroprevalence study was conducted in 2017, including 348 equids from eastern Austria. Serum samples reactive by ELISA for either flavivirus immunoglobulin G or M were further analyzed with the plaque reduction neutralization test (PRNT-80) to identify the specific etiologic agent. Neutralizing antibody prevalences excluding vaccinated equids were found to be 5.3% for WNV, 15.5% for TBEV, 0% for USUV, and 1.2% for WNV from autochthonous origin. Additionally, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to detect WNV nucleic acid in horse sera and was found to be negative in all cases. Risk factor analysis did not identify any factors significantly associated with seropositivity.

引用

页数：16

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