Indirect determination of pyruvic acid by capillary electrophoresis with amperometric detection

被引：8

作者：

Lu, Xin ^{[1
]}

Huang, Wei-Hua ^{[1
]}

Ai, Feng ^{[1
]}

Wang, Zong-Li ^{[1
]}

Cheng, Jie-Ke ^{[1
]}

机构：

[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 857卷 / 02期

关键词：

capillary electrophoresis; amperometric detection; carbon fiber microelectrode; hydroxylamine; pyruvic acid;

D O I：

10.1016/j.jchromb.2007.07.042

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method of indirectly measuring pyruvic acid (PA) by capillary electrophoresis with amperometric detection is proposed for the first time. It is based on the oximation reaction between PA and hydroxylamine (NH2OH), and the quantification of PA was performed by direct and sensitive amperometric detection of excessive NH2OH after the oximation reaction. This method displayed a good sensitivity, and the detection limits of NH2OH and PA are 1.76 x 10(-7) and 3.88 x 10(-7) mol/L, respectively at S/N = 3. The linear relationship between the peak current and PA concentration is exhibited over the range from 4 x 10(-6) to 1 X 10(-4) mol/L. This method has been applied to determine PA in rat plasma with satisfactory results. (c) 2007 Published by Elsevier B.V.

引用

页码：347 / 351

页数：5

共 29 条

[1] Rapid on-line microdialysis hyphenated technique for the dynamic monitoring of extracellular pyruvate, lactic acid and ascorbic acid during cerebral ischemia [J].