The catalytic activity of cytochrome P450 3A22 is critical for the metabolism of T-2 toxin in porcine reservoirs

被引：23

作者：

Ge, Xiaohu ^{[1
]}

Wang, Junping ^{[1
]}

Liu, Jing ^{[1
]}

Jiang, Jun ^{[1
]}

Lin, Huina ^{[1
]}

Wu, Jun ^{[1
]}

Ouyang, Man ^{[1
]}

Tang, Xianqing ^{[1
]}

Zheng, Ming ^{[1
]}

Liao, Ming ^{[2
]}

Deng, Yiqun ^{[1
]}

机构：

[1] S China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China

[2] S China Agr Univ, Minist Agr China, Key Lab Anim Dis Control & Prevent, Guangzhou 510642, Guangdong, Peoples R China

来源：

CATALYSIS COMMUNICATIONS | 2010年 / 12卷 / 02期

关键词：

Enzyme catalysis; Cytochrome P450; Toxin; Metabolism; DRUG-METABOLISM; INDUCED APOPTOSIS; DNA-DAMAGE; TOXICITY; PATHWAYS; CHONDROCYTES; MYCOTOXINS; CELLS; LIVER;

D O I：

10.1016/j.catcom.2010.08.003

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Contamination of food by T 2 toxin a trichothecene mycotoxin poses a serious threat to human health We report the identification of cytochrome P450 3A22 (CYP3A22) responsible for 3' hydroxylation of T-2 and HT-2 in pigs The present study shows that T-2 toxin significantly induces CYP3A22 expression in primary piglet hepatocytes Moreover recombinant pig CYP3A22 converted T-2 toxin into TC-1 (3'-OH T-2 toxin) and HT 2 toxin into TC-3 (3'-OH HT-2 toxin) in vitro These results suggest that pig CYP3A22 primarily eliminates T-2 and HT-2 toxins by 3'-hydroxylation of isovaleryl groups Therefore CYP3A22 is critical for xenobiotic metabolism and endogenous biochemical biotransformation of trichothecene mycotoxin in porcine reservoirs (C) 2010 Elsevier B V All rights reserved

引用

页码：71 / 75

页数：5

共 25 条

[1]

Atroshi F, 1997, MOL ASPECTS MED, V18, pS255

[2] Cytotoxicity and related effects of T-2 toxin on cultured Vero cells [J].

Bouaziz, C. ;

Abid-Essefi, S. ;

Bouslimi, A. ;

El Golli, E. ;

Bacha, H. .

TOXICON, 2006, 48 (03) :343-352

[3] T-2 toxin induces apoptosis, and selenium partly blocks, T-2 toxin induced apoptosis in chondrocytes through modulation of the Bax/Bcl-2 ratio [J].

Chen, JH ;

Chu, YL ;

Cao, JL ;

Yang, ZT ;

Guo, X ;

Wang, ZL .

FOOD AND CHEMICAL TOXICOLOGY, 2006, 44 (04) :567-573

[4] T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes [J].

Chen, Jing-hong ;

Cao, Jun-ling ;

Chu, Yong-lie ;

Wang, Zhi-lun ;

Yang, Zhan-tian ;

Wang, Hong-lin .

JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B, 2008, 9 (06) :455-463

[5]

Dohnal V, 2008, CURR DRUG METAB, V9, P77

[6]

Donato MT, 2008, CURR DRUG METAB, V9, P1

[7] METABOLIC-ACTIVATION OF AFLATOXIN-B1 - PATTERNS OF DNA ADDUCT FORMATION, REMOVAL, AND EXCRETION IN RELATION TO CARCINOGENESIS [J].

ESSIGMANN, JM ;

CROY, RG ;

BENNETT, RA ;

WOGAN, GN .

DRUG METABOLISM REVIEWS, 1982, 13 (04) :581-602

[8] The role of dietary nucleotides in reduction of DNA damage induced by T-2 toxin and deoxynivalenol in chicken leukocytes [J].

Frankic, T. ;

Pajk, T. ;

Rezar, V. ;

Levart, A. ;

Salobir, J. .

FOOD AND CHEMICAL TOXICOLOGY, 2006, 44 (11) :1838-1844

[9] Cytochrome P-450 3A4: Regulation and role in drug metabolism [J].

Guengrich, FP .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1999, 39 :1-17

[10] IDENTIFICATION OF T-2 TOXIN IN MOLDY CORN ASSOCIATED WITH A LETHAL TOXICOSIS IN DAIRY-CATTLE [J].

HSU, IC ;

SMALLEY, EB ;

STRONG, FM ;

RIBELIN, WE .

APPLIED MICROBIOLOGY, 1972, 24 (05) :684-690

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