Single-molecule detection on a portable 3D-printed microscope

被引：42

作者：

Brown, James W. P. ^{[1
,2
]}

Bauer, Arnaud ^{[1
,2
]}

Polinkovsky, Mark E. ^{[1
,2
]}

Bhumkar, Akshay ^{[1
,2
]}

Hunter, Dominic J. B. ^{[3
]}

Gaus, Katharina ^{[1
,2
,4
]}

Sierecki, Emma ^{[1
,2
]}

Gambin, Yann ^{[1
,2
]}

机构：

[1] Univ New South Wales, EMBL Australia Node Single Mol Sci, Sydney, NSW 2052, Australia

[2] Univ New South Wales, Sch Med Sci, Sydney, NSW 2052, Australia

[3] Univ Queensland, Inst Mol Biosci, St Lucia, Qld 4072, Australia

[4] Univ New South Wales, ARC Ctr Excellence Adv Mol Imaging, Sydney, NSW 2052, Australia

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

澳大利亚研究理事会; 英国医学研究理事会;

关键词：

ALPHA-SYNUCLEIN; AGGREGATION; COMPLEX;

D O I：

10.1038/s41467-019-13617-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Single-molecule assays have, by definition, the ultimate sensitivity and represent the next frontier in biological analysis and diagnostics. However, many of these powerful technologies require dedicated laboratories and trained personnel and have therefore remained research tools for specialists. Here, we present a single-molecule confocal system built from a 3D-printed scaffold, resulting in a compact, plug and play device called the AttoBright. This device performs single photon counting and fluorescence correlation spectroscopy (FCS) in a simple format and is widely applicable to the detection of single fluorophores, proteins, liposomes or bacteria. The power of single-molecule detection is demonstrated by detecting single alpha-synuclein amyloid fibrils, that are currently evaluated as biomarkers for Parkinson's disease, with an improved sensitivity of >100,000-fold over bulk measurements.

引用

页数：7

共 37 条

[31] Ultracompact alignment-free single molecule fluorescence device with a foldable light path [J].

Singh, Niraj Kumar ;

Chacko, Jenu V. ;

Sreenivasan, Varun K. A. ;

Nag, Suman ;

Maiti, Sudipta .

JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (02)

[32] Stoichiometry and Affinity of Thioflavin T Binding to Sup35p Amyloid Fibrils [J].

Sulatskaya, Anna I. ;

Kuznetsova, Irina M. ;

Belousov, Mikhail V. ;

Bondarev, Stanislav A. ;

Zhouravleva, Galina A. ;

Turoverov, Konstantin K. .

PLOS ONE, 2016, 11 (05)

[33] Extrinsic Amyloid-Binding Dyes for Detection of Individual Protein Aggregates in Solution [J].

Taylor, Christopher G. ;

Meisl, Georg ;

Horrocks, Mathew H. ;

Zetterberg, Henrik ;

Knowles, Tuomas P. J. ;

Klenerman, David .

ANALYTICAL CHEMISTRY, 2018, 90 (17) :10385-10393

[34] Plasmonics Enhanced Smartphone Fluorescence Microscopy [J].

Wei, Qingshan ;

Acuna, Guillermo ;

Kim, Seungkyeum ;

Vietz, Carolin ;

Tseng, Derek ;

Chae, Jongjae ;

Shir, Daniel ;

Luo, Wei ;

Tinnefeld, Philip ;

Ozcan, Aydogan .

SCIENTIFIC REPORTS, 2017, 7

[35] In vivo demonstration that α-synuclein oligomers are toxic [J].

Winner, Beate ;

Jappelli, Roberto ;

Maji, Samir K. ;

Desplats, Paula A. ;

Boyer, Leah ;

Aigner, Stefan ;

Hetzer, Claudia ;

Loher, Thomas ;

Vilar, Marcal ;

Campioni, Silvia ;

Tzitzilonis, Christos ;

Soragni, Alice ;

Jessberger, Sebastian ;

Mira, Helena ;

Consiglio, Antonella ;

Pham, Emiley ;

Masliah, Eliezer ;

Gage, Fred H. ;

Riek, Roland .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (10) :4194-4199

[36] Single-Molecule Studies in Live Cells [J].

Yu, Ji .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 67, 2016, 67 :565-585

[37] Determination of equilibrium and rate constants for complex formation by fluorescence correlation spectroscopy supplemented by dynamic light scattering and Taylor dispersion analysis [J].

Zhang, Xuzhu ;

Poniewierski, Andrzej ;

Jelinska, Aldona ;

Zagozdzon, Anna ;

Wisniewska, Agnieszka ;

Hou, Sen ;

Holyst, Robert .

SOFT MATTER, 2016, 12 (39) :8186-8194

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