m6aViewer: software for the detection, analysis, and visualization of N6-methyladenosine peaks from m6A-seq/ME-RIP sequencing data

被引：32

作者：

Antanaviciute, Agne ^{[1
]}

Baquero-Perez, Belinda ^{[2
,3
]}

Watson, Christopher M. ^{[4
]}

Harrison, Sally M. ^{[1
]}

Lascelles, Carolina ^{[1
]}

Crinnion, Laura ^{[4
]}

Markham, Alexander F. ^{[1
]}

Bonthron, David T. ^{[1
]}

Whitehouse, Adrian ^{[2
,3
]}

Carr, Ian M. ^{[1
]}

机构：

[1] Univ Leeds, St Jamess Univ Hosp, Inst Biomed & Clin Sci, Sect Genet,Sch Med, Leeds LS9 7TF, W Yorkshire, England

[2] Univ Leeds, Fac Biol Sci, Sch Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England

[3] Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England

[4] St James Univ Hosp, Yorkshire Reg Genet Serv, Leeds LS9 7TF, W Yorkshire, England

来源：

RNA | 2017年 / 23卷 / 10期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

m(6)A; RNA methylation; peak-calling; m(6)A-seq; next-generation sequencing; MESSENGER-RNA METHYLATION; MODEL; IDENTIFICATION; MICRORNAS; BROWSER;

D O I：

10.1261/rna.058206.116

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent methods for transcriptome-wide N-6-methyladenosine (m(6)A) profiling have facilitated investigations into the RNA methylome and established m(6)A as a dynamic modification that has critical regulatory roles in gene expression and may play a role in human disease. However, bioinformatics resources available for the analysis of m(6)A sequencing data are still limited. Here, we describe m6aViewer-a cross-platform application for analysis and visualization of m(6)A peaks from sequencing data. m6aViewer implements a novel m(6)A peak-calling algorithm that identifies high-confidence methylated residues with more precision than previously described approaches. The application enables data analysis through a graphical user interface, and thus, in contrast to other currently available tools, does not require the user to be skilled in computer programming. m6aViewer and test data can be downloaded here: http://dna2.leeds.ac.uk/m6a.

引用

页码：1493 / 1501

页数：9

共 40 条

[1] N6-methyladenosine marks primary microRNAs for processing [J].