Metabolic response of porcine colon explants to in vitro infection by Brachyspira hyodysenteriae: a leap into disease pathophysiology

Introduction Swine dysentery caused by Brachyspira hyodysenteriae is a production limiting disease in pig farming. Currently antimicrobial therapy is the only treatment and control method available. Objective The aim of this study was to characterize the metabolic response of porcine colon explants to infection by B. hyodysenteriae. Methods Porcine colon explants exposed to B. hyodysenteriae were analyzed for histopathological, metabolic and pro-inflammatory gene expression changes. Results Significant epithelial necrosis, increased levels of l-citrulline and IL-1 alpha were observed on explants infected with B. hyodysenteriae. Conclusions The spirochete induces necrosis in vitro likely through an inflammatory process mediated by IL-1 alpha and NO.