Analysis of RNA-protein interactions by flow cytometry

被引：0
|
作者：
Brodsky, AS
Johnston, AP
Trau, M
Silver, PA
机构：
[1] Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[3] Univ Queensland, Dept Chem, Ctr Nanotechnol & Biomat, St Lucia, Qld 4072, Australia
来源：
CURRENT OPINION IN MOLECULAR THERAPEUTICS | 2003年 / 5卷 / 03期
关键词：
flow cytometry; functional genomics; microbeads; microspheres; RNA-protein interactions;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.
引用
收藏
页码：235 / 240
页数：6
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