Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol

被引：130

作者：

Gao, Jian ^{[1
,2
]}

Xu, Hong ^{[1
]}

Li, Qiu-jie ^{[2
]}

Feng, Xiao-hai ^{[1
]}

Li, Sha ^{[1
]}

机构：

[1] Nanjing Univ Technol, Coll Food Sci & Light Ind, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Peoples R China

[2] Yancheng Inst Technol, Sch Chem & Biol Engn, Yancheng 224051, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 18期

关键词：

Inulin; Jerusalem artichoke tubers; One-step fermentation; Paenibacillus polymyxa ZJ-9; R,R-2,3-butanediol; MICROBIAL-PRODUCTION; ASPERGILLUS-NIGER; 2,3-BUTANEDIOL; GLUCOSE; ACID;

D O I：

10.1016/j.biortech.2010.03.143

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The medium for one-step fermentation of raw inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol (R,R-2,3-BD) was developed Inulin, K2HPO4 and NH4Cl were found to be the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration was further investigated according to the Box-Behnken design and determined to be 77.14 g/L, 3.09 g/L and 0.93 g/L, respectively Under the optimal conditions, the concentration of the obtained R,R-2,3-BD was 36 92 g/L, at more than 98% optical purity Compared with other investigated carbon resources, fermentation of the raw inulin extract afforded the highest yield of R,R-2,3-BD This process featured one-step fermentation of inulin without further hydrolyzing, which greatly decreased the raw material cost and thus facilitated its practical application. (C) 2010 Elsevier Ltd. All rights reserved

引用

页码：7076 / 7082

页数：7

共 31 条

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