Site-Specific 5-Formyl Cytosine Mediated DNA-Histone Cross-Links: Synthesis and Polymerase Bypass by Human DNA Polymerase η

被引：6
作者：
Pujari, Suresh S. [1 ]
Wu, Mingxuan [2 ,3 ,4 ]
Thomforde, Jenna [1 ]
Wang, Zhipeng A. [2 ]
Chao, Christopher [1 ]
Olson, Noelle M. [5 ]
Erber, Luke [1 ]
Pomerantz, William C. K. [5 ]
Cole, Philip [2 ]
Tretyakova, Natalia Y. [1 ]
机构：
[1] Univ Minnesota, Coll Pharm, Dept Med Chem, Minneapolis, MN 55455 USA
[2] Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[3] Westlake Univ, Sch Sci, 18 Shilongshan Rd, Hangzhou 310024, Zhejiang, Peoples R China
[4] Westlake Inst Adv Study, Inst Nat Sci, 18 Shilongshan Rd, Hangzhou 310024, Zhejiang, Peoples R China
[5] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2021年 / 60卷 / 51期
关键词：
5-formyl-dC; DNA-protein cross-link; histone; oxime ligation; polymerase bypass; POSTTRANSLATIONAL MODIFICATIONS; REPAIR; REPLICATION; COMPLEXES; PROTEINS;
D O I：
10.1002/anie.202109418
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
DNA-protein cross-links (DPCs) between DNA epigenetic mark 5-formylC and lysine residues of histone proteins spontaneously form in human cells. Such conjugates are likely to influence chromatin structure and mediate DNA replication, transcription, and repair, but are challenging to study due to their reversible nature. Here we report the construction of site specific, hydrolytically stable DPCs between 5fdC in DNA and K4 of histone H3 and an investigation of their effects on DNA replication. Our approach employs oxime ligation, allowing for site-specific conjugation of histones to DNA under physiological conditions. Primer extension experiments revealed that histone H3-DNA crosslinks blocked DNA synthesis by hPol eta polymerase, but were bypassed following proteolytic processing.
引用
收藏
页码：26489 / 26494
页数：6
相关论文
共 41 条
  • [1] DNA-protein crosslinks: their induction, repair, and biological consequences
    Barker, S
    Weinfeld, M
    Murray, D
    [J]. MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2005, 589 (02) : 111 - 135
  • [2] Structure and mechanism of human DNA polymerase η
    Biertuempfel, Christian
    Zhao, Ye
    Kondo, Yuji
    Ramon-Maiques, Santiago
    Gregory, Mark
    Lee, Jae Young
    Masutani, Chikahide
    Lehmann, Alan R.
    Hanaoka, Fumio
    Yang, Wei
    [J]. NATURE, 2010, 465 (7301) : 1044 - U102
  • [3] Post-Translational Modifications of Histones That Influence Nucleosome Dynamics
    Bowman, Gregory D.
    Poirier, Michael G.
    [J]. CHEMICAL REVIEWS, 2015, 115 (06) : 2274 - 2295
  • [4] Repair of a DNA-Protein Crosslink by Replication-Coupled Proteolysis
    Duxin, Julien P.
    Dewar, James M.
    Yardimci, Hasan
    Walter, Johannes C.
    [J]. CELL, 2014, 159 (02) : 346 - 357
  • [5] Proteolytic Degradation of Topoisomerase II (Top2) Enables the Processing of Top2.DNA and Top2.RNA Covalent Complexes by Tyrosyl-DNA-Phosphodiesterase 2 (TDP2)
    Gao, Rui
    Schellenberg, Matthew J.
    Huang, Shar-yin N.
    Abdelmalak, Monica
    Marchand, Christophe
    Nitiss, Karin C.
    Nitiss, John L.
    Williams, R. Scott
    Pommier, Yves
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (26) : 17960 - 17969
  • [6] Repair and biochemical effects of DNA-protein crosslinks
    Ide, Hiroshi
    Shoulkamy, Mahmoud I.
    Nakano, Toshiaki
    Miyamoto-Matsubara, Mayumi
    Salem, Amir M. H.
    [J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2011, 711 (1-2) : 113 - 122
  • [7] Tet Proteins Can Convert 5-Methylcytosine to 5-Formylcytosine and 5-Carboxylcytosine
    Ito, Shinsuke
    Shen, Li
    Dai, Qing
    Wu, Susan C.
    Collins, Leonard B.
    Swenberg, James A.
    He, Chuan
    Zhang, Yi
    [J]. SCIENCE, 2011, 333 (6047) : 1300 - 1303
  • [8] Age-related increases of 8-hydroxy-2′-deoxyguanosine and DNA-protein crosslinks in mouse organs
    Izzotti, A
    Cartiglia, C
    Taningher, M
    De Flora, S
    Balansky, R
    [J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 1999, 446 (02) : 215 - 223
  • [9] 5-Formylcytosine mediated DNA-protein cross-links block DNA replication and induce mutations in human cells
    Ji, Shaofei
    Fu, Iwen
    Naldiga, Spandana
    Shao, Hongzhao
    Basu, Ashis K.
    Broyde, Suse
    Tretyakova, Natalia Y.
    [J]. NUCLEIC ACIDS RESEARCH, 2018, 46 (13) : 6455 - 6469
  • [10] Reversible DNA-Protein Cross-Linking at Epigenetic DNA Marks
    Ji, Shaofei
    Shao, Hongzhao
    Han, Qiyuan
    Seiler, Christopher L.
    Tretyakova, Natalia Y.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2017, 56 (45) : 14130 - 14134
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