Heterologous Expression of Aspergillus oryzae Xylose Reductase and Xylitol Dehydrogenase Genes Facilitated Xylose Utilization in the Yeast Saccharomyces cerevisiae

被引：4
|
作者：
Kaneda, Jitsuro [1 ]
Sasaki, Kenji [1 ]
Gomi, Katsuya [1 ]
Shintani, Takahiro [1 ]
机构：
[1] Tohoku Univ, Dept Bioind Informat & Genom, Grad Sch Agr Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2011年 / 75卷 / 01期
关键词：
Aspergillus oryzae; Saccharomyces cerevisiae; xylose fermentation; xylose reductase; ethanol; FERMENTATION; IMPROVEMENT;
D O I：
10.1271/bbb.100639
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cDNA encoding a putative xylose reductase (xyrA) from Aspergillus oryzae was cloned and coexpressed in the yeast Saccharomyces cerevisiae with A. oryzae xylitol dehydrogenase cDNA (xdhA). XyrA exhibited NADPH-dependent xylose reductase activity. The S. cerevisiae strain, overexpressing the xyrA, xdhA, endogenous XKS1, and TAL1 genes, grew on xylose as sole carbon source, and produced ethanol.
引用
收藏
页码：168 / 170
页数：3
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