Heterologous Expression of Aspergillus oryzae Xylose Reductase and Xylitol Dehydrogenase Genes Facilitated Xylose Utilization in the Yeast Saccharomyces cerevisiae

被引：4

作者：

Kaneda, Jitsuro ^{[1
]}

Sasaki, Kenji ^{[1
]}

Gomi, Katsuya ^{[1
]}

Shintani, Takahiro ^{[1
]}

机构：

[1] Tohoku Univ, Dept Bioind Informat & Genom, Grad Sch Agr Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2011年 / 75卷 / 01期

关键词：

Aspergillus oryzae; Saccharomyces cerevisiae; xylose fermentation; xylose reductase; ethanol; FERMENTATION; IMPROVEMENT;

D O I：

10.1271/bbb.100639

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA encoding a putative xylose reductase (xyrA) from Aspergillus oryzae was cloned and coexpressed in the yeast Saccharomyces cerevisiae with A. oryzae xylitol dehydrogenase cDNA (xdhA). XyrA exhibited NADPH-dependent xylose reductase activity. The S. cerevisiae strain, overexpressing the xyrA, xdhA, endogenous XKS1, and TAL1 genes, grew on xylose as sole carbon source, and produced ethanol.

引用

页码：168 / 170

页数：3

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