Isolation of renal proximal tubular brush-border membranes

被引：105
作者：
Biber, Juerg [1 ]
Stieger, Bruno [2 ]
Stange, Gerti [1 ]
Murer, Heini [1 ]
机构：
[1] Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland
[2] Univ Zurich Hosp, Dept Med, Div Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland
来源：
NATURE PROTOCOLS | 2007年 / 2卷 / 06期
关键词：
D O I：
10.1038/nprot.2007.156
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.
引用
收藏
页码：1356 / 1359
页数：4
相关论文
共 14 条
  • [1] A HIGH-YIELD PREPARATION FOR RAT-KIDNEY BRUSH-BORDER MEMBRANES - DIFFERENT BEHAVIOR OF LYSOSOMAL MARKERS
    BIBER, J
    STIEGER, B
    HAASE, W
    MURER, H
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 647 (02) : 169 - 176
  • [2] Booth A G, 1974, Biochem J, V142, P575
  • [3] Essential role for collectrin in renal amino acid transport
    Danilczyk, Ursula
    Sarao, Renu
    Remy, Christine
    Benabbas, Chahira
    Stange, Gerti
    Richter, Andreas
    Arya, Sudha
    Pospisilik, J. Andrew
    Singer, Dustin
    Camargo, Simone M. R.
    Makrides, Victoria
    Ramadan, Tamara
    Verrey, Francois
    Wagner, Carsten A.
    Penninger, Josef M.
    [J]. NATURE, 2006, 444 (7122) : 1088 - 1091
  • [4] PROPERTIES OF BRUSH-BORDER VESICLES ISOLATED FROM RAT-KIDNEY CORTEX BY CALCIUM PRECIPITATION
    EVERS, C
    HAASE, W
    MURER, H
    KINNE, R
    [J]. MEMBRANE BIOCHEMISTRY, 1978, 1 (3-4): : 203 - 219
  • [5] RABBIT SMALL INTESTINAL BRUSH-BORDER MEMBRANE PREPARATION AND LIPID-COMPOSITION
    HAUSER, H
    HOWELL, K
    DAWSON, RMC
    BOWYER, DE
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 602 (03) : 567 - 577
  • [6] SIMPLE PROCEDURE FOR ISOLATION OF RAT LIVER MICROSOMES
    KAMATH, SA
    KUMMEROW, FA
    NARAYAN, KA
    [J]. FEBS LETTERS, 1971, 17 (01) : 90 - &
  • [7] MODIFIED PROCEDURE FOR RAPID PREPARATION OF EFFICIENTLY TRANSPORTING VESICLES FROM SMALL INTESTINAL BRUSH-BORDER MEMBRANES - THEIR USE IN INVESTIGATING SOME PROPERTIES OF D-GLUCOSE AND CHOLINE TRANSPORT-SYSTEMS
    KESSLER, M
    ACUTO, O
    STORELLI, C
    MURER, H
    MULLER, M
    SEMENZA, G
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 506 (01) : 136 - 154
  • [8] KINNESAFFRAN E, 1989, METHOD ENZYMOL, V172, P3
  • [9] Kriz W., 1992, KIDNEY PHYSL PATHOPH, V3, P587
  • [10] LEVER JE, 1982, J BIOL CHEM, V257, P8680
12