Neural crest-derived multipotent cells in the adult mouse iris stroma

被引：31

作者：

Kikuchi, Miki ^{[1
]}

Hayashi, Ryuhei ^{[1
]}

Kanakubo, Sachiko ^{[1
,2
]}

Ogasawara, Ayumi ^{[2
]}

Yamato, Masayuki ^{[3
]}

Osumi, Noriko ^{[2
]}

Nishida, Kohji ^{[1
]}

机构：

[1] Tohoku Univ, Sch Med, Dept Ophthalmol, Sendai, Miyagi 980, Japan

[2] Tohoku Univ, Sch Med, Ctr Translat & Adv Anim Res, Div Dev Neurosci,Aoba Ku, Sendai, Miyagi 9808574, Japan

[3] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Tokyo, Japan

来源：

GENES TO CELLS | 2011年 / 16卷 / 03期

关键词：

STEM-CELLS; FATE; EYE; DIFFERENTIATION;

D O I：

10.1111/j.1365-2443.2011.01485.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The purpose of this study was to characterize neural crest-derived cells within the adult murine iris. The iris was isolated from P0-Cre/Floxed-EGFP transgenic (TG) mice. The isolated iris cells formed EGFP-positive spheres on non-adhesive culture plates. Immunostaining showed that these EGFP-positive spheres expressed neural crest markers including Sox10 and p75NTR, and these cells showing in vitro sphere-forming ability were originally resided in the iris stroma (IS), in vivo. Real-time RT-PCR showed that the EGFP-positive spheres expressed significantly higher levels of the neural crest markers than EGFP-negative spheres and bone marrow-derived mesenchymal stem cells. Furthermore, the iris stromal sphere had capability to differentiate into various cell lineages including smooth muscle and cartilage. These data indicate that neural crest-derived multipotent cells can be isolated from the murine IS and expanded in sphere culture.

引用

页码：273 / 281

页数：9

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