Cloning of murine low molecular weight phosphotyrosine protein phosphatase cDNA: identification of a new isoform

被引：16

作者：

Magherini, F ^{[1
]}

Giannoni, E ^{[1
]}

Raugei, G ^{[1
]}

Cirri, P ^{[1
]}

Paoli, P ^{[1
]}

Modesti, A ^{[1
]}

Camici, G ^{[1
]}

Ramponi, G ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Sci Biochim, I-50134 Florence, Italy

来源：

FEBS LETTERS | 1998年 / 437卷 / 03期

关键词：

phosphotyrosine protein phosphatase; alternative splicing; mouse;

D O I：

10.1016/S0014-5793(98)01241-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The low molecular weight phosphotyrosine protein phosphatase (LMW-PTP) is a 18 kDa cytosolic enzyme, involved in the negative regulation of cell proliferation. In different mammalian species LMW-PTPs are expressed in two molecular forms produced from a single primary transcript through an alternative splicing mechanism. In this paper we report the cloning, expression and characterization of mouse isoforms of LMW-PTPs (called m-IF1 and m-IF2), very similar to the corresponding rat and human isoenzymes, Moreover we have identified a third cDNA encoding a protein (m-IF2P) that presents three substitutions compared to m-IF2, This new isoform is still active on pNPP, although to a lower extent: this reduction is mainly due to the leucine to proline substitution in position 13, within the catalytic loop. The mRNA expression level of this isoform is comparable to those of m-IF1 and m-IF2, It is likely that a gene duplication process followed by mutations has generated this new gene. (C) 1998 Federation of European Biochemical Societies.

引用

页码：263 / 266

页数：4

共 21 条

[1] CRYSTAL-STRUCTURE OF HUMAN PROTEIN-TYROSINE-PHOSPHATASE 1B [J].