Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)

被引：26

作者：

Afrin, Khandker Shazia ^{[1
]}

Rahim, Md Abdur ^{[1
,2
]}

Park, Jong-In ^{[1
]}

Natarajan, Sathishkumar ^{[1
]}

Kim, Hoy-Taek ^{[1
]}

Nou, Ill-Sup ^{[1
]}

机构：

[1] Sunchon Natl Univ, Dept Hort, Sunchon 57922, South Korea

[2] Sher & Bangla Agr Univ, Dept Genet & Plant Breeding, Dhaka 1207, Bangladesh

来源：

MOLECULAR BIOLOGY REPORTS | 2018年 / 45卷 / 05期

关键词：

Black rot; Cabbage; Resistance; NBS-encoding genes; Expression; CAMPESTRIS PV. CAMPESTRIS; GENOME-WIDE ANALYSIS; XANTHOMONAS-CAMPESTRIS; ARABIDOPSIS-THALIANA; EXPRESSION PATTERNS; LRR GENES; CRUCIFERS; INHERITANCE; INFECTION; PATHOVARS;

D O I：

10.1007/s11033-018-4217-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heading cabbage is a nutritionally rich and economically important cruciferous vegetable. Black rot disease, caused by the bacterium Xanthomonas campestris pv. campestris, reduces both the yield and quality of the cabbage head. Nucleotide binding site (NBS)-encoding resistance (R) genes play a vital role in the plant immune response to various pathogens. In this study, we analyzed the expression and DNA sequence variation of 31 NBS-encoding genes in cabbage (Brassica oleracea var. capitata). These genes encoded TIR, NBS, LRR and RPW8 protein domains, all of which are known to be involved in disease resistance. RNA-seq revealed that these 31 genes were differentially expressed in leaf, root, silique, and stem tissues. Furthermore, qPCR analyses revealed that several of these genes were more highly expressed in resistant compared to susceptible cabbage lines, including Bol003711, Bol010135, Bol010559, Bol022784, Bol029866, Bol042121, Bol031422, Bol040045 and Bol042095. Further analysis of these genes promises to yield both practical benefits, such as molecular markers for marker-assisted breeding, and fundamental insights to the mechanisms of resistance to black rot in cabbage.

引用

页码：773 / 785

页数：13

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