Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles

被引：40

作者：

Cetin, Meltem

Aktas, Yesim

Vural, Imran

Capan, Yilmaz ^{[1
]}

Dogan, Lale A.

Duman, Memed

Dalkara, Turgay

机构：

[1] Hacettepe Univ, Fac Pharm, Dept Pharmaceut Technol, TR-06100 Ankara, Turkey

[2] Hacettepe Univ, Fac Med, Dept Basic Oncol, TR-06100 Ankara, Turkey

[3] Hacettepe Univ, Inst Oncol, Ankara, Turkey

[4] Hacettepe Univ, Dept Chem Engn, TR-06532 Ankara, Turkey

[5] Hacettepe Univ, Bioengn Div, Ankara, Turkey

[6] Hacettepe Univ, Fac Med, Dept Neurol, TR-06100 Ankara, Turkey

来源：

DRUG DELIVERY | 2007年 / 14卷 / 08期

关键词：

bFGF; chitosan; nanoparticles; SDS-PAGE; TPP;

D O I：

10.1080/10717540701606483

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions.

引用

页码：525 / 529

页数：5

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