Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles

被引：40

作者：

Cetin, Meltem

Aktas, Yesim

Vural, Imran

Capan, Yilmaz ^{[1
]}

Dogan, Lale A.

Duman, Memed

Dalkara, Turgay

机构：

[1] Hacettepe Univ, Fac Pharm, Dept Pharmaceut Technol, TR-06100 Ankara, Turkey

[2] Hacettepe Univ, Fac Med, Dept Basic Oncol, TR-06100 Ankara, Turkey

[3] Hacettepe Univ, Inst Oncol, Ankara, Turkey

[4] Hacettepe Univ, Dept Chem Engn, TR-06532 Ankara, Turkey

[5] Hacettepe Univ, Bioengn Div, Ankara, Turkey

[6] Hacettepe Univ, Fac Med, Dept Neurol, TR-06100 Ankara, Turkey

来源：

DRUG DELIVERY | 2007年 / 14卷 / 08期

关键词：

bFGF; chitosan; nanoparticles; SDS-PAGE; TPP;

D O I：

10.1080/10717540701606483

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions.

引用

页码：525 / 529

页数：5

共 50 条

[1] Preparation, Characterization, and In Vitro Evaluation of EGCG-loaded Chitosan Nanoparticles
Li, JiaLei
Zu, YuanGang
Zhao, XiuHua
An, ZhiGang
Sui, XiaoYu
Wang, KunLun
Sun, XiaoLi
ADVANCED RESEARCH ON MATERIAL ENGINEERING, CHEMISTRY AND BIOINFORMATICS, PTS 1 AND 2 (MECB 2011), 2011, 282-283 : 539 - 544
[2] bFGF-loaded HA-chitosan: A promising scaffold for periodontal tissue engineering
Akman, Abdullah C.
Tigli, R. Seda
Gumusderelioglu, Menemse
Nohutcu, Rahime M.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2010, 92A (03) : 953 - 962
[3] Preparation and in vitro/in vivo evaluation of resveratrol- loaded carboxymethyl chitosan nanoparticles
Zu, Yuangang
Zhang, Yin
Wang, Weiguo
Zhao, Xiuhua
Han, Xue
Wang, Kunlun
Ge, Yunlong
DRUG DELIVERY, 2016, 23 (03) : 981 - 991
[4] Methotrexate loaded chitosan nanoparticles: Preparation, radiolabeling and in vitro evaluation for breast cancer diagnosis
Ekinci, Meliha
Ilem-Ozdemir, Derya
Gundogdu, Evren
Asikoglu, Makbule
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 2015, 30 : 107 - 113
[5] Preparation of Atorvastatin Loaded Chitosan Nanoparticles: In Vitro and In Vivo Evaluations
Lavanya, V.
Rajeswari, P.
Vidyavathi, M.
Sureshkumar, R. V.
INTERNATIONAL JOURNAL OF NANOSCIENCE, 2020, 19 (05)
[6] bFGF-Loaded Mesoporous Silica Nanoparticles Promote Bone Regeneration Through the Wnt/?-Catenin Signalling Pathway
Shen, Mingkui
Wang, Lulu
Feng, Li
Gao, Yi
Li, Sijing
Wu, Yulan
Xu, Chuangye
Pei, Guoxian
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2022, 17 : 2593 - 2608
[7] Preparation, characterization and in vitro evaluation of 5-fluorouracil loaded into chitosan-acacia gum nanoparticles
Al-Nasrawi, Hasan
Shalan, Naeem
Abualsoud, Bassam M.
Nsairat, Hamdi
THERAPEUTIC DELIVERY, 2024, 15 (05) : 339 - 353
[8] bFGF-Loaded Mesoporous Silica Nanoparticles Promote Bone Regeneration Through the Wnt/β-Catenin Signalling Pathway
Shen, Mingkui
Wang, Lulu
Feng, Li
Gao, Yi
Li, Sijing
Wu, Yulan
Xu, Chuangye
Pei, Guoxian
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2022, 17 : 2593 - 2608
[9] Preparation, Characterization and Evaluation of Bromocriptine Loaded Chitosan Nanoparticles for Intranasal Delivery
Md, Shadab
Kumar, Manish
Baboota, Sanjula
Sahni, Jasjeet Kaur
Ali, Javed
SCIENCE OF ADVANCED MATERIALS, 2012, 4 (09) : 949 - 960
[10] Levocetirizine Dihydrochloride-Loaded Chitosan Nanoparticles: Formulation and In Vitro Evaluation
Yurtdas Kirimlioglu, Gulsel
Ozturk, A. Alper
TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES, 2020, 17 (01) : 27 - 35

← 1 2 3 4 5 →