Kinetics of cytokine expression in bovine PBMCs and whole blood after in vitro stimulation with foot-and-mouth disease virus (FMDV) antigen

被引：13

作者：

Dar, Pervaiz A. ^{[1
,2
]}

Hajam, Irshad A. ^{[1
]}

Suryanarayana, Velavurthy S. ^{[1
]}

Kishore, Subodh ^{[1
]}

Kondabattula, Ganesh ^{[1
]}

机构：

[1] IVRI, FMD Res Ctr, Bangalore 560024, Karnataka, India

[2] SKUAST Kashmir, Fac Vet Sci & Anim Husb, Div Vet Microbiol & Immunol, Srinagar 190006, Jammu & Kashmir, India

来源：

CYTOKINE | 2015年 / 72卷 / 01期

关键词：

Foot-and-mouth disease virus; Cattle; Interleukin (IL)-2; IL-4; Interferon (IFN)-gamma; T-CELL CLONE; IMMUNE-RESPONSES; VACCINE; QUANTIFICATION; PROTECTION;

D O I：

10.1016/j.cyto.2014.12.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interest in analysing antigen-specific cytokine responses has substantially increased in recent years, in part due to their use in assessing vaccine efficacy. In the present study, the kinetics of IL-2, IL-4 and IFN-gamma expression was determined in bovine PBMCs by real-time PCR and in whole blood by cytokine-release assay after in vitro stimulation with recall foot-and-mouth disease virus (FMDV) antigen. The results showed that the cytokine mRNA of IL-2 and IFN-gamma in PBMCs were induced early (peak induction at 6 h), whereas the IL-4 mRNA showed delayed induction (peaked at 24 h). In contrast, the kinetics of cytokine proteins in whole blood was different and required the accumulation of the proteins before being optimally detected. The peak accumulation of cytokine protein in whole blood was recorded at 72 h for IL-2 and IL-4, and 96 h for IFN-gamma. The findings of this study are of importance when selecting an optimal time points for measuring antigen-specific cytokine expression in cattle. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：58 / 62

页数：5

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