Sugar sensing responses to low and high light in leaves of the C4 model grass Setaria viridis

被引:22
|
作者
Henry, Clemence [1 ]
Watson-Lazowski, Alexander [1 ]
Oszvald, Maria [2 ]
Griffiths, Cara A. [2 ]
Paul, Matthew [2 ]
Furbank, Robert T. [3 ]
Ghannoum, Oula [1 ]
机构
[1] Western Sydney Univ, Hawkesbury Inst Environm, ARC Ctr Excellence Translat Photosynth, Locked Bag 1797, Penrith, NSW 2751, Australia
[2] Rothamsted Res, Plant Sci, Harpenden AL5 2JQ, Herts, England
[3] Australian Natl Univ, ARC Ctr Excellence Translat Photosynth, Res Sch Biol, Acton, ACT 2601, Australia
基金
英国生物技术与生命科学研究理事会;
关键词
C-4; photosynthesis; glucose; hexokinase (HXK); Setaria viridis; sucrose; sucrose-non fermenting 1 (Snf1)-related protein kinase 1 (SnRK1); sugar signalling; target of rapamycin (TOR); trehalose-6-phosphate; MAIZE LEAF PHOTOSYNTHESIS; GENE-EXPRESSION; PLANT-GROWTH; SIGNAL-TRANSDUCTION; METABOLIC PATHWAYS; DIURNAL CHANGES; SINK-REGULATION; STRESS; HEXOKINASE; SEQUENCE;
D O I
10.1093/jxb/erz495
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Although sugar regulates photosynthesis, the signalling pathways underlying this process remain elusive, especially for C-4 crops. To address this knowledge gap and identify potential candidate genes, we treated Setaria viridis (C-4 model) plants acclimated to medium light intensity (ML, 500 mu mol m(-2) s(-1)) with low (LL, 50 mu mol m(-2) s(-1)) or high (HL, 1000 mu mol m(-2) s(-1)) light for 4 d and observed the consequences on carbon metabolism and the transcriptome of source leaves. LL impaired photosynthesis and reduced leaf content of signalling sugars (glucose, sucrose, and trehalose-6-phosphate). In contrast, HL strongly induced sugar accumulation without repressing photosynthesis. LL more profoundly impacted the leaf transcriptome, including photosynthetic genes. LL and HL contrastingly altered the expression of hexokinase (HXK) and sucrose-non-fermenting 1 (Snf1)-related protein kinase 1 (SnRK1) sugar sensors and trehalose pathway genes. The expression of key target genes of HXK and SnRK1 were affected by LL and sugar depletion, while surprisingly HL and strong sugar accumulation only slightly repressed the SnRK1 signalling pathway. In conclusion, we demonstrate that LL profoundly impacted photosynthesis and the transcriptome of S. viridis source leaves, while HL altered sugar levels more than LL. We also present the first evidence that sugar signalling pathways in C-4 source leaves may respond to light intensity and sugar accumulation differently from C-3 source leaves.
引用
收藏
页码:1039 / 1052
页数:14
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